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分子信标用于核酸连续复制过程的体外实时监测 被引量：4

Real-time Monitoring Continuous Replication of DNA Leading Strand in vitro Based on Molecular Beacon
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摘要 利用分子信标核酸探针实时监测了核酸连续复制过程.分子信标不仅作为模板参与复制反应,而且同步将复制过程的信息转换为荧光信号,实现复制过程的体外实时监测.该方法不仅为DNA复制检测提供了一种实时研究手段,而且为核酸复制动力学及与复制相关疾病的深入研究提供了一种新的思路. Real-time monitoring of replication of DNA leading strand using molecular beacon in vitro was reported. DNA replication plays a pivotal role in life process. DNA replication is usually assayed by denatured gel electrophoresis and autoradiography, which are complex, time-consuming, and incapable of acquiring the dynamic data in real time. We employed molecular beacon as the probe and template for DNA replication and converted directly replication information into fluorescence signal. The replication information of DNA leading strand was obtained conveniently and accurately using molecular beacon. This method would provide a strategy for real-time monitoring DNA replication and for further research of replication dynamic data and some deseases associated with DNA replication.
作者 孟祥贤 羊小海 王柯敏 郭秋平 李军 唐志文
机构地区 湖南大学化学生物传感与计量学国家重点实验室
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2009年第8期1538-1542,共5页 Chemical Journal of Chinese Universities
基金 国家自然科学基金(批准号:20505007,90606003) 湖南省自然科学基金(批准号:08JJ1002)资助
关键词 分子信标 核酸复制 实时监测 荧光信号 Molecular beacon DNA replication Real-time monitoring Fluorescent signal
分类号 O657 [理学—分析化学]
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参考文献29

  • 1Watson J. D., CrickF. H.. Nature[J], 1953, 171(4361): 964--967.
  • 2Watson J. D., Crick F. H.. Nature[J], 1953, 171(4356) : 737--738.
  • 3Meselson M., Stahl F. W.. Proc. Natl. Acad. Sci. USA[J], 1958, 44:671--682.
  • 4Okazaki R. , Okazaki T. , Sakabe K.. Proc. Natl. Acad. Sci. USA[J] , 1968, 59:598-605.
  • 5CHENYue-Zeng(陈阅增).General Biology(普通生物学)[M],Bering:Higher Education Press,2001:412-436.
  • 6Berndt M. , Jane B. , Carmen F. , et al.. J. Cell Biol. [J], 2007, 179:138--1398.
  • 7Tonatiuh R. S., Irina P., Olga L., et al.. Nucleic Acids Res. [J], 2009, 37(1) : 38--46.
  • 8Tsurimoto T. , Stiillman B.. J. Biol. Chem.[J] , 1991,266(3) : 1950--1960.
  • 9Minden J. S. , Marians K. J.. J. Biol. Chem. [J] , 1985, 260(16) : 9316-9325.
  • 10Huang T. ,Chen C. W.. J. Bacteriol. [J], 2008, 190:755-758.

二级参考文献37

  • 1Guerrier T, Altman S. Catalytic activity of an RNA molecule prepared by transcription in vitro. Science, 1984, 223:285-286.
  • 2Phylactou L A. Ribozyme gene therapy. Methods Mol Biol, 2001,175:521-529.
  • 3Watanabe T, Sullenger B A. Induction of wild-type p53 activity inhuman cancer cells by ribozymes that repair mutant p53 transcripts. Proc Natl Acad Sci USA, 2000, 97(15): 8490-8494.
  • 4Lewin A S, hauswirth W W. Ribozyme gene therapy: applications for molecular medicine. Trends Mol Med, 2001, 7(5): 221-228.
  • 5Wang D Y, Lai B H, Feldman A R, et al. A general approach for the use oligonucleotide effectors to regulate the catalysis of RNA-cleaving ribozymes and DNAzymes. Nucleic Acids Res,2002, 30(8): 1735-1742.
  • 6Esteban J A, Walter N G, Kotzorek G, et al. Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme. Proc Natl Acad Sci USA, 1998, 95:6091-6096.
  • 7Singh K K, Rcker T, Hanne A, et al. Fluorescence polarization for monitoring ribozyme reactions in real time. Biotechniques, 2000.29(2): 344-348.
  • 8Kirk S R, Luedtke N W, Tor Y. 2-Aminopurine as a real-time probe of enzymatic cleavage and inhibition of hammerhead ribozymes. Bioorg Med Chem, 2001, 9(9): 2295-2301.
  • 9Singh K K, Parwaresch R, Krupp G. Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET). RNA, 1999, 5:1348-1356.
  • 10Tyagi S, Kramer F R. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotech, 1996, 14:303-308.

共引文献6

同被引文献43

  • 1何春梅,刘斌,王柯敏,唐红星,何丽芳,李惠敏.p53及p21^(waf1)蛋白表达与人舌癌细胞多药耐药性的关系[J].中国医师杂志,2006,8(9):1189-1192. 被引量：5
  • 2LIJun(李军) WANGKe-Min(王柯敏) TANWei-Hong(谭蔚泓)etal.高等学校化学学报,2004,25(3):421-424.
  • 3Syvanen A.C..Nature Reviews Genetics[J],2001,2(12):930-942.
  • 4Wang K.,Tang Z.,Yang C.J.,Kim Y.,Fang X.,Li W.,Wu Y.,Medley C.D.,Cao Z.,Li J.,Colon P.,Lin H.,Tan W..Angew.Chem.Int.Ed.[J],2009,48(5):856-870.
  • 5Zuo X.,Yang X.,Wang K.,Tan W.,Wen J..Anal.Chim.Acta[J],2007,587(1):9-13.
  • 6Tang Z.,Wang K.,Tan W.,Li J.,Liu L.,Guo Q.,Meng X.,Ma C.,Huang S..Nucleic Acids Res.[J],2003,31(23):e148.
  • 7Tang Z.,Wang K.,Tan W.,Ma C.,Li J.,Liu L.,Guo Q.,Meng X..Nucleic Acids Res.[J],2005,33(11):e97.
  • 8Ma C.,Tang Z.,Wang K.,Tan W.,Yang X.,Li W.,Li Z.,Lü X..Anal.Biochem.[J],2007,363(2):294-296.
  • 9Bratu D.P.,Cha B.J.,Mhlanga M.M.,Kramer F.R.,Tyagi S..Proc.Natl.Acad.Sci.[J],2003,100(26):13308-13313.
  • 10Rodriguez A.J.,Condeelis J.,Singer R.H.,Dictenberg J..Semin.Cell Dev.Biol.[J],2007,18(2):202-208.

引证文献4

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高等学校化学学报
2009年 第8期

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