醇类分子诱导的肌血球素构象变化

Alcohol-induced Conformation of Myoglobin

系统研究了各种醇类分子对肌血球素构象变化的影响.结果表明,在pH=4,V(醇)/V(水)=30/70的溶液中,各种不同的醇类分子可以导致肌血球素构象的变化,且这种变化与各种醇的不同分子结构有关,如醇的烷基和结构变化会导致不同程度蛋白质分子构象的变化,并呈现出一定的规律性.随着直链烷基基团的增大,醇分子对蛋白质分子结构的诱导变性能力提高;仅改变醇分子结构时几乎产生相同的诱导效应,但会带来蛋白质分子内离子配位和电子自旋态的改变;用氟原子取代醇分子上的氢原子,在一定程度上对于hMb和aMb具有选择性的稳定作用.实验方法包括电喷雾电离质谱、圆二色谱、和紫外光谱等.

This paper mainly reports the alcohol-induced effection. Accoding to the mass spectrometry, we got a lot differences among myoglobin conformations induced by different alcohol at pH = 4.0, 30% （volume fraction） alcohol. The results show that alcohol＇s molecular structures effect Mb conformation. Besides, we found that the alkyl＇s number enhanced denaturation effection. Fluorine substituent could stabilize hMb and aMb selectively a certain extent, we also proved the regularity summarized as above farther and got more useful information of Fe ion＇s coordination and election spin state via CD spectroscopy and UV-Vis Soret Absorption.