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香烟烟雾提取物致人气道平滑肌细胞损伤及对白介素-8表达的影响 被引量：3

Effects of cigarette smoke extract on human airway smooth muscle cells and on the expression of interleukin-8 in them
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摘要 目的:观察香烟烟雾提取物(cigarette smoke extract,CSE)对人气道平滑肌细胞(human airway smooth muscle cells,HASMCs)的损伤及对白介素-8(interleukin-8,IL-8)表达水平的影响。方法:原代培养HASMCs,分为空白对照组、CSE组,在干预24h后使用倒置显微镜、MTT法观察HASMCs的活性,用ELISA法和RT-PCR法测定IL-8表达水平的变化。结果:CSE可导致HASMCs形态和结构的改变,同时使HASMCs分泌释放IL-8增加。结论:在香烟烟雾提取物刺激下,HASMCs分泌IL-8增加。 Objective To investigate the effects of cigarette smoke extract （CSE） on human airway smooth muscle cells （HASMC） and on the expression of interleukin-8 （IL-8） in them. Method The primary cultured HASMC were divided into blank control group and CSE group. Then the activity of HASMC was observed by invert microscope and MTT method, and the expression of IL-8 was measured by ELISA and RT-PCR 24 h after intervention. Result CSE can cause morphologic and structural changes of HASMC and increase the expression of IL-8 in HASMC. Conclusion Under the stimulation of CSE, expression of IL-8 is increased in HASMC.
作者 肖卫 刘辉国 蕾茹 胡伟华 竺红宇 陈振平 许菊秀 马家兰 黄晓霞
机构地区 长江大学附属第一医院呼吸内科 华中科技大学同济医学院附属同济医院呼吸内科
出处 《实用医学杂志》 CAS 北大核心 2009年第16期2620-2622,共3页 The Journal of Practical Medicine
关键词 肌细胞 平滑肌 白细胞介素8 香烟烟雾提取物 Myocytes, smooth muscle Interleukin-8 Cigarette smoke extract
分类号 R563.9 [医药卫生—呼吸系统]
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参考文献7

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同被引文献34

  • 1付强,成静,韩中博,李小兰,陈小燕,张鹏,肖徽,陶德定,胡俊波,龚建平.香烟烟雾提取物对人呼吸道上皮细胞DNA损伤和凋亡的影响[J].癌症,2006,25(10):1191-1197. 被引量：8
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  • 10Sexton K, Balharry D, Brennan P, et al. Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components. Biomarkers, 2011,16 :567-576.

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实用医学杂志
2009年 第16期

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