父婴传播乙型肝炎病毒在胎盘绒毛中的表达分析

Expression of hepatitis B virus in placental villus of 89 cases with the paternal-fetal transmission of hepatitis B virus

目的:探讨在父系HBsAg阳性的流产胚胎中HBV-DNA感染和表达情况。方法:在仅男方感染乙型肝炎病毒HBsAg阳性组合,即女HBsAg(-)且男HBsAg(+)流产组合中,按以下组合将入选对象分为4组,组1为女HBsAb(+)且男大三阳HBeAg(+),组2为女HBsAb(+)且男小三阳HBeAg(-),组3为女HBsAb(-)且男大三阳HBeAg(+),组4为女HBsAb(-)且男小三阳HBeAg(-)。观察其胚胎绒毛HBV-DNA表达情况。结果:男方感染乙型肝炎病毒HBsAg(+)89例组合中,仅在男大三阳HBeAg(+)组合发现绒毛HBV-DNA升高。女HBsAb(-)且男HBeAg(+)组合中2例,女HBsAb(+)且男HBeAg(+)组合中1例。女方HBsAb阳性与否在男方大三阳组中子代绒毛HBV-DNA升高率差异无显著性(P>0.05)。结论:大三阳父亲更容易发生父婴垂直传播,女方HBsAb阳性与否不能完全阻断父婴垂直传播。

Objective To investigate the expression of HBV-DNA in abortive embryo with paternal HBsAg- positive. Methods Couples of men with serum HBsAg（＋） and women with HBsAg（-） were divided into 4 groups： Group 1 were couples of women with HBsAb（＋） and men with HBeAg（＋）, Group 2 were women with HBsAb（＋） and men with HBeAg（-）, Group 3 were women with HBsAb（-） and men with HBeAg（＋）, Group 4 were women with HBsAb（-） and men with HBeAg（-）. The expression of HBV-DNA in placental villus was detected. Results In all 89 cases, HBV-DNA in placental villus were increased in couples of men with HBeAg（＋）, those were 2 cases in couples of women with HBsAb （-） and men with HBeAg（＋） and 1 cases in couples of women with HBsAb（＋） and men with HBeAg（＋）. There was no significant effect of whether the women with HBsAb （＋） or not on the expression of HBV-DNA in placental villus （P 〉 0.05）. Conclusions Paternal-fetal transmission of hepatitis B virus is more prone to happen in couples of men with HBeAg（＋）, and on which whether the women with HBsAb（＋） or not has no significant effect.