二色补血草的组织培养和离体快繁研究

Study on Tissue Culture and Rapid Propagation in Vitro of Limonium bicolor

[目的]为二色补血草的快速繁殖技术提供科学指导。[方法]以二色补血草的子叶与胚轴作为外植体,建立快速繁殖体系,研究不同激素浓度对诱导愈伤组织、不定芽以及生根率的影响。[结果]不同激素浓度的培养基均能使外植体不同程度地生成愈伤组织及不定芽;生根培养基中只有A、B、H、I组有不定根产生,其余组均无不定根产生。[结论]以子叶为外植体,愈伤组织和不定芽的最佳诱导培养基分别为:MS+0.7 mg/L6-BA+0.1 mg/LNAA、MS+0.5 mg/L6-BA+0.1 mg/LNAA;以胚轴为外植体,愈伤组织和不定芽的最佳诱导培养基为:MS+0.3 mg/L6-BA+0.5 mg/LNAA。诱导不定根的最佳培养基为:MS0。

[ Objective ] The study aimed to provide the scientific direction of rapid propagation technique. [ Method] Taking leaf and hypoeotyl of Limonium bicolor as the explant, the system of rapid propagation were established, the influences of hormone concentration to induce the tissue culture and adventitious buds were studied. [ Result] Different concentration culture medium can induce the explants grew out tissue culture and adventitious buds, A, B, H, I groups of rooting medium can induce adventitious buds generated adventitious roots, the other groups had no adventitious roots. [ Conclusion ] Taking cotyledons as explant, the best induction medium of tissue culture and adventitious buds were MS0 ＋ 0.7 mg/L 6-BA ＋ 0.1 mg/L NAA, MS0 ＋ 0.5 mg/L 6-BA ＋ 0. 1 mg/L NAA respectively ; with hypocotyl as explant, the best induction medium of tissue culture and adventitious buds was MS0 ＋ 0.3 mg/L 6-BA ＋ 0.5 mg/L NAA. The best medium for inducing adventitious roots was MSgr.