当归多糖水提法正交设计及最佳Savag’s法去蛋白工艺

The Orthogonal Design of Chinese Angelica Polysaccharide with Water Extraction and the Optimum Process of Removing Protein with Method of Savag's

[目的]筛选出传统水提法提取当归多糖的最佳工艺及Savag’s法去蛋白时最佳操作次数。[方法]运用正交设计法设计提取工艺;采用Savag’s法去蛋白,并比较不同次数对多糖含量的影响。总糖含量测定采用改良苯酚-硫酸法;蛋白质含量测定采用考马斯亮蓝法;糖醛酸含量测定采用硫酸-咔唑法。[结果]提取的最佳工艺为:料水比1∶20、提取时间30 min、提取温度100℃;最佳去蛋白条件为:氯仿-正丁醇(5∶1),操作3次。[结论]该方法简便、易操作,适用于水溶性粗多糖的提取和检测。

[ Objective ] The study aimed to select the best process of Chinese Angelica Polysaccharide （CAPS） with water extraction and the optimum operating times of removing protein with method of Savag＇ s. [ Method] The orthogonal design was used to extract of CAPS. The Savag＇ s method was used to remove protein from polysaccharide and the polysaccharide contents were compared after the different removing times. The total polysaccharide contents were determined with method of phenol-sulfuric acid, the protein content determined by method of Coomassie brilliant blue, the uronic acid content with method of sulfate-carbazole. [ Result ] The optimum extracting process was that ratio of material to water was 1：20, the extraction time was 30 minutes, extraction temperature was 100℃ and the optimum process of removing protein was 3 times （ 5：1 ratio of chloroform ： n-butanol ）. [ Conclusion ] The method was simple and easy to operate and was suitable for extracting and detecting of water-soluble polysaccharide .