粉防己碱对大鼠脑缺血再灌注后脑组织钙离子和氧自由基的作用

Effects of Tetrandrine on calcium ion and oxygen free radicals in brain tissue after ischemia-reperfusion in rats

目的探讨粉防己碱(Tet)对脑缺血再灌注后脑组织钙离子超载、氧自由基损伤机制的作用。方法72只SD大鼠随机分为假手术组、模型组和粉防己碱组。采用线栓法复制大鼠右侧大脑中动脉脑缺血再灌注模型,各组于缺血再灌注12、24h进行神经功能缺损评分后,取出右侧大脑,测定脑组织含水量、钙离子含量、超氧化物歧化酶(SOD)和丙二醛(MDA)。结果模型组与假手术组相比,脑组织含水量、钙离子含量及MDA水平显著升高(P<0.01),SOD水平明显降低(P<0.01),神经功能缺损明显(P<0.001);粉防己碱组与模型组相比,脑组织含水量、钙离子含量及MDA水平均显著降低(P<0.01或P<0.05),SOD水平明显增高(P<0.01),神经功能缺损明显减轻(P<0.05)。结论脑缺血再灌注早期应用粉防己碱,可通过拮抗钙离子内流,抑制钙离子超载;以及通过增强缺血脑组织SOD活力,降低MDA含量,从而起到清除氧自由基、减轻再灌注损伤、保护脑细胞的作用。

[Objective] To explore the effect of Tetrandrine on calcium ion and oxygen free radicals in brain tissue after cerebral isehemia-reperfusion in rats. [Methods] The 72 healthy SD adult were selected, and were randomly divided into sham-operated group（Sham）, cerebral ischemia-reperfusion group （Model） and Tetrandrine treating group（Tet）. Cerebral ischemia-reperfusion model was established by occlusion of the right middle cerebral artery 2 hours than removal of the occlusion. Neurological deficits seores（NDSs） were measured and the levels of water, calcium ion, SOD, MDA were determinded at 12 hours, 24 hours after isehemia-reperfusion respectively. [Results] The levels of water, NDSs, calcium ion, MDA in brain tissue at different time point of Model group were significantly higher but the activity of SOD was lower compared with those of Sham group （P 〈0.01）; The levels of water, NDSs, calcium ion, MDA in brain tissue at different time point of Tet group were significantly lower but the activity of SOD was higher compared with those of Model group（P 〈0.05 or P 〈0.01）. [Conclusion] The administration of Tet at early stage of cerebral ischemia-repeffusion increased the SOD activities and decreased the levels of MDA, then eliminating oygen free radicals and reducing reperfusion injuries.