普乐林对K562、K562/AO2的增殖抑制和耐药逆转作用

Effects of Puerarin suppresses K562 and K562/AO2 cells growth and reverse the multidrug resistance of K562/AO2

目的研究黄酮类化合物普乐林(Puerarin)对K562和K562/AO2细胞增殖抑制、药物增敏、耐药逆转的作用。方法台盼蓝拒染试验、MTT实验检测Puerarin对K562、K562/AO2的抑制作用;MTT实验检测阿霉素单独和与Puerarin合用对K562、K562/AO2的半数抑制浓度。结果3.2μmol/L的Puerarin即对K562、K562/AO2有生长抑制作用,其最大抑制浓度为25.0μmol/L,Puerarin对两种细胞的抑制差异无统计学意义(P>0.05);阿霉素对K562细胞的IC50为0.131μg/mL,对K562/AO2的IC50为7.542μg/mL,耐药倍数为57.14倍;Puerarin加大了阿霉素对K562的杀伤敏感性(最大增敏倍数1.42),同时能部分逆转K562/AO2对阿霉素的耐药性(最大相对逆转效率91%)。结论Puerarin对K562、K562/AO2细胞的增殖抑制作用有限;但Puerarin具有较明显逆转K562/AO2对阿霉素的耐药作用。

[Objective] To explore whether the Puerarin, one kind of Flavonoids, can inhibit the proliferation of the K562 and K562/AO2 cell lines and reverse the mutidrug resistance （MDR） of K562/AO2. [Methods] The inhibition of Puerarin to the two cell lines was detected by trypan blue exclusion test, MTr assay; IC50 was detected with MTT assay of the two cell lines incubated with ADM independently or associated with Puerarin. [Results] The Puerarin demonstrated some inhibition to the two cell lines, there was no significant difference between the two cell lines （P 〉0.05）. TheIC50 of the ADM to K562 is 0.131 μg/mL, and to the K562/AO2 is 7.542 μg/mL, the resistant fold is 57.136. Puerarin promote sensibility of the two cell lines to ADM, the sensitization efficiency is 1.422 fold to the K562, and the relative reversal efficiency to the K562/AO2 is 91%. [Conclusion] Puerarin inhibits the two cell lines proliferating but not very effective, maybe it means the inhibition of Puerarin has high selectity to tumors cells, but it reverse the drug-resistance of K562/AO2 to ADM significantly.