七氟烷对老年大鼠海马毒蕈碱受体M2表达和认知功能的影响

Effect of Sevoflurone on the expression of M2 acetylcholine receptor in the hippocampus and cognitive of aged rats

目的探讨不同剂量七氟烷对老年大鼠海马毒蕈碱受体M2受体亚型(mAChRM2)表达的影响及与认知功能的关系。方法40只SD大鼠随机分为空白对照组、1.5%sevⅠ组、1.5%sevⅡ组、3.0%sevⅠ组和3.0%sevⅡ组,每组8只。采用Y型迷宫进行学习记忆能力测试,通过RT-PCR法和免疫组织化学法测定大鼠海马mAChRM2表达。结果与对照组相比,3.0%sevⅠ组学习记忆能力下降(P<0.05),而且海马mAChRM2表达显著减少(P<0.05)。其余各组差异无显著性。结论给予大鼠七氟烷麻醉时,大鼠学习记忆能力随七氟烷剂量加大,在麻醉后1d学习记忆能力减弱,可能引起了短时POCD,这些可能与大鼠海马mAChRM2减少有关。

[Objective] To investigate the effect of Sevoflurone on the expression of M2 aeetylcholine receptor in the hippocampus and the cognition of aged rats. [Methods] 40 SD rats weighting 550-600 g were randomly divided into 5 groups （n=8）： the normal control group; 1.5% see Ⅰ group（be tested after received 1.5% Sevoflurone 1 day）; 1.5% see Ⅱgroup （be tested after received 1.5% sevoflurone 7 days）; 3.0%see Ⅰ（be tested after received3.0% Sevoflurone 1 day） and 3.0% sev group （be tested after received 3.0% Sevoflurone 7 days）. Y-maze was used to test the ability of learning and memory on rats.The expression of M2 acetylcholine receptor （ mAChR M2） in the hippocampus of rats were tested by RT-PCR and immunhistochemistry. [Results] The ability of learning and memory consolidation in 3.0% see Ⅰ group were declined compared with that in the normal control group （P 〈0.05）. And the mAChR M2 expression levels in hippocampus of 3.0% see I group were fewer than those in normal control group rats （P 〈0.05）. The other three groups were immunhistochemistry compared with the normal control group. [Conclusion] The abilty of learning and memory consolidation were declined with the Sevoflurone dose increased after one day by Sevoflumne anesthetized .The transient POCD was caused by Sevoflurone. Suggesting mAChR M2 exepression levels declined may contribute to these phenomenons.