电离辐射旁效应的研究方法概述

Advances in research methods for radiation-induced bystander effects

无论是放射性核素内照射还是不同射线外照射,无论是辐照体外培养细胞还是活体照射,都存在一定的辐射旁效应。旁效应的研究方法很多,如低剂量常规辐照,未照射细胞与照射细胞共同培养,用辐照细胞条件培养基处理未辐照细胞,微束单细胞定点辐照等。本文就辐射旁效应的研究方法做一综述。

Either internal radiation using radionuclide or external radiation using different rays induced bystander effects have been confirmed certainly by use of the cell cultured in vitro or in vivo exposed to radiation. There are many methods to study the bystander effects, such as conventional low-dose radiation, un-irradiated cells co-cultured with irradiated cells, treatment with irradiated cells conditioned media, single-cell micro-beam irradiation, and so on. Whichever method is used depends on the laboratory conditions and research purposes. A series of summary has been made in this paper.