阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究

The comparative study on screening of pleurotus polysaccharide high-yield strains by use of ion beam implantation and composite mutagenesis

为筛选出阿魏菇菌丝体多糖高产菌株,本文进行了阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究。尝试用打散的阿魏菇菌丝体为靶材,以离子束注入为诱变手段,通过营养缺陷型初筛和摇瓶发酵复筛,获得了2株阿魏菇多糖高产菌株PFPH-1和PFPH-2。其菌丝体多糖产量分别达到551.80mg/L和659.46mg/L,比出发菌株分别提高了46.55%和75.14%。再分别以PFPH-1和PFPH-2为出发菌株,用紫外线和氯化锂进行复合诱变处理,通过高通量初筛和摇瓶发酵复筛,结果发现,菌株1、9和10号比其出发菌株PFPH-1的多糖产量分别减少了27%、38%和37%;菌株17号比其出发菌株PFPH-2的多糖产量减少了28%。在本研究中,紫外线和氯化锂的复合诱变表现出了一定的负效应。

In order to screen pleurotus mycelium polysaccharide high-yield strains, the comparative study was made by use of ion beam implantation and composite mutagenesis before screening.The treating mycelium pellet of pleurotus ferulae tentatively with ion beam implantation was performed at the first. Two polysacchafide high-yield strains, PFPH-land PFPH-2, were selected using fermentation quantitative screening after anxotrophy qualitative primary screening. It has been found that the polysaccharide yield of the mutants is 551.80mg/L and 659.46mg/L respectively, which increases by 46.55% and 75.14% respectively compared to that of initial strain. Then PFPH-land PFPH-2, as the original strain, is exposed to ultraviolet light and is suffered by additive of LiCl respectively. The results indicate that the polysaccharide yield of strains 1, 9 and 10 decreases by 27%, 38% and 37% respectively compared to that of PFPH-1 meanwhile the polysaccharide yield of strain 17 decreases by 28% compared to that of PFPH-2 after high-flux qualitative primary screening. In this study, composite mutagenesis with exposure of ultraviolet and additive of lithium chloride shows some negative effects.