翼状胬肉中糖皮质激素受体的表达及意义

Expression and significance of glucocorticoid receptor in pterygium

目的检测糖皮质激素受体-α、α（GR—α、GR—β）在翼状胬肉组织中的表达，探讨其在翼状胬肉发病机制中的作用。方法取39例翼状胬肉组织（其中初发30例，复发9例），应用荧光定量逆转录PCR和免疫组化技术检测GR—α、βmRNA和蛋白表达，并选择8例正常人的球结膜做为对照。结果翼状胬肉及正常人的球结膜组织中均存在GR-αmRNA表达，但GR-βmRNA表达均为阴性。其中39例翼状胬肉中的GR—αmRNA为（10．40±1．42）×10^3拷贝／μg，8例正常眼球结膜中GR—αmRNA为（2．23±0．32）×10^3拷贝／μg，差异有统计学意义（t=4．82，P〈0．01）。其中初发性胬肉（30例）表达为（10．40±0．40）×10^3拷贝／μg，而复发性胬肉（9例）为（10．50±0．30）×10^3拷贝／μg，较初发性胬肉略高，但差异无统计学意义（t=0．55，P〉0．05）。结论翼状胬肉组织中GR—αmRNA及其蛋白表达明显增高，这可能是翼状胬肉发生、发展的重要原因之一，但其作用机制尚待于进一步研究。

Objective To investigate whether theglucocorticoid receptor would be expressed in pterygium and discuss its significant. Methods GR -α and GR -β mRNA expression were detected by a real -time fluorescent quantitative RT - PCR. GR - α and GR - β protein expression were examined by Western - blotting analysis and immunohistochemical technique from 39 patients with pterygium （30 primary pterygium, 9 recurrent pterygium）. Normal conjunctivae（n =8） were as controls. Results The expression of GR - α mRNA were （ 10. 40 ± 1.42 ） × 10^3 GR - α cDNA copies/μg total RNA in pterygium and （2.23 ± 0. 32） × 10^3GR-α cDNA copies/μg total RNA in normal conjunctivae. The expression of GR- β mRNA was not detected in pterygium and in normal conjunctivae. There was no difference between the expression of GR - α mRNA in recurrent pterygium（ 10.50 ± 0.30） and the primary pterygium（ 10.40 ± 0.40） （ P 〉 0.05 ）. There was significantly difference between the expression of GR - α mRNA in pterygium and the normal conjunctivae. Western - blotting analysis and immunohistochemical technique confirmed these findings for the protein transcripts of these genes. Conclusions These results demonstrate that the expression of GR - α mRNA and protein are higher in pterygium and probably are one of the important reasons. But its effection and mechanismin pterygium still need more investigate.