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过量表达腺苷甲硫氨酸合成酶基因能提高转基因烟草中多聚胺的生物合成 被引量：4

Overexpression of S-adenosylmethionine Synthetase Promote Polyamine Biosynthesis in Transgenic Tobacco
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摘要 以土壤农杆菌介导的转化方法将盐地碱蓬的腺苷甲硫氨酸合成酶cDNA(SsSAMS2)转化到烟草K326中。PCR分析的结果表明,SsSAMS2整合进K326的基因组内。共筛选到8个转基因纯合品系,分析其中3个品系(ST8-9、ST14-2、ST3-5)基因表达和多聚胺含量的结果表明,SsSAMS2可在转基因烟草中表达,转基因烟草中的多聚胺含量明显高于野生型烟草。这些结果表明,腺苷甲硫氨酸合成酶基因已在转基因烟草中过量表达并导致多聚胺含量提高。 The full-length S-adenosylmethionine synthetase （SsSAMS2） of Suadea salsa was introduced into tobacco （Nicotiana tabacum L. K326） by Agrobacterium tumefaciens-mediated transformation. The gene integration in tobacco was confirmed by PCR. Eight homozygous transgenic lines （ST lines） were screened. The result of SsSAMS2 expression and polyamines （PAs） content analysis of three lines （ST8-9, ST14-2, ST3-5） showed that SsSAMS2 expressed in transgenic tobacco, and transgenic tobacco accumulated more soluble total PAs content than wild-type plants. These results indicated that the promotion of soluble total PAs content resulted from the SsSAMS2 overexpression in transgenic plants.
作者 戚元成 马雷 王菲菲 刘卫群
机构地区 河南农业大学烟草学院 河南农业大学生命科学学院
出处 《植物生理学通讯》 CSCD 北大核心 2009年第8期791-793,共3页 Plant Physiology Communications
基金 国家"863"计划项目(2002AA629080) 国家烟草专卖局重点项目(110200001011A)
关键词 腺苷甲硫氨酸合成酶 多聚胺 转基因烟草 S-adenosylmethionine synthetase polyamine transgenic tobacco
分类号 S572 [农业科学—烟草工业]
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参考文献15

  • 1戚元成,张世敏,王丽萍,王明道,张慧.谷胱甘肽转移酶基因过量表达能加速盐胁迫下转基因拟南芥的生长[J].植物生理与分子生物学学报,2004,30(5):517-522. 被引量：30
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二级参考文献13

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共引文献29

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二级引证文献9

植物生理学通讯
2009年 第8期

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