P53、P21^(WAF1)和CDK1在卵巢上皮性癌组织中的表达及意义

Expression and significance of P53, P21^(WAF1) and CDK1 proteins in epithelial ovarian cancer

背景与目的:P53、P21WAF1和CDK1是细胞周期中G2/M期DNA损伤检验点"P53通路"的关键因子,本研究拟探讨P53、P21WAF1和CDK1在卵巢上皮性癌组织中的表达及意义。方法:应用免疫组织化学技术检测20例正常卵巢组织、20例卵巢良性上皮性肿瘤组织、76例卵巢上皮性癌组织中P53、P21WAF1、CDK1蛋白的表达,并分析卵巢上皮性癌组织中P53、P21WAF1、CDK1蛋白表达与其临床病理特征的关系以及各蛋白表达之间的相关性。结果:P53、P21WAF1和CDK1蛋白在卵巢上皮性癌中的阳性率与正常卵巢组织和良性卵巢肿瘤相比其差异均有统计学意义(P<0.05),而在正常卵巢组织与良性卵巢肿瘤之间其阳性率差异均无统计学意义(P>0.05)。在卵巢上皮性癌中,临床分期越晚、组织分化越差,P53蛋白表达越高;随着临床分期的增加,P21WAF1蛋白表达逐渐降低;CDK1蛋白的表达与各临床病理特征无相关性。卵巢上皮性癌中P53蛋白和P21WAF1蛋白的表达与CDK1蛋白的表达呈负相关(r=-0.388,P=0.001;r=-0.282,P=0.014),P53蛋白与CDK1蛋白表达呈正相关(r=0.263,P=0.022)。结论:P53蛋白与卵巢上皮性癌的恶性程度相关,P53和P21WAF1蛋白可能与卵巢上皮性癌的恶性进展有关。检测CDK1可能有助于卵巢癌的早期诊断。

Background and Objective; P53, P21^WAF1 and CDK1 are key factors in the ＂P53 pathway＂ of GSM phase DNA damage checkpoint in cell cycle. This study was to investigate the expression and significance of P53, P21^WAF1 and CDK1 proteins in epithelial ovarian cancer. Methods：The expression of P53, P21^WAF1 and CDK1 proteins in 20 specimens of normal ovarian tissues, 20 specimens of benign epithelial ovarian tumor and 76 specimens of epithelial ovarian cancer was detected by immunohistochemistry. Their correlations to the clinicopathologic characteristics of epithelial ovarian cancer and their interrelationships were analyzed. Results： Significant differences were noted in the positive rates of P53, P21^WAF1 and CDK1 proteins between epithelial ovarian cancer and normal ovarian tissues, benign ovarian tumors （P 〈 0.05）. In epithelial ovarian cancer, up-regulation of P53 protein was associated with advanced FIGO stage and poor differentiation; down-regulation of P21^WAF1 protein was associated with advanced FIGO stage; CDK1 showed no association with any clinicopathologic factors. P53 and CDK1 expression were negatively correlated to P21^WAF1 expression （r = -0.388, P = 0.001;r = -0.282, P = 0.014）; P53 expression was positively correlated to CDK1 expression （r = 0.263, P = 0.022）. Conclusions： P53 protein is related to the malignancy of epithelial ovarian cancer, P53 and P21^WAF1 protein may be related to the malignant development of epithelial ovarian cancer. CDK1 detection may be helpful in the early diagnosis of epithelial ovarian cancer.