姜黄素抗肝纤维化的实验研究

Prophylactic Effects of Curcumin on Rats Hepatic Fibrosis

目的:探讨姜黄素的抗肝纤维化作用。方法:将大鼠随机分为正常对照组、模型组、高、中、低3个剂量姜黄素组和阳性对照组,用皮下注射CC l4法诱导大鼠肝纤维化。测量并计算肝脏指数;测定各组大鼠ALT及AST活性以评价肝功能;同时观察各组大鼠肝脏形态学变化。结果:模型组大鼠肝脏指数均明显低于正常对照组(P<0.01),姜黄素治疗组大鼠肝脏指数明显高于模型组大鼠(P<0.05)。与正常组大鼠比较,模型组大鼠肝脏AST和ALT均显著升高(P<0.01),给予姜黄素治疗后,各治疗组大鼠肝脏AST和ALT活性均显著下降(P<0.05)。HE染色及M asson三色染色显示,秋水仙碱治疗组及各剂量姜黄素治疗组可见肝损伤病变明显减轻,纤维组织增生程度也明显轻于模型组。结论:姜黄素能明显减轻CC l4所致肝损伤,保护肝脏正常结构和功能,延缓肝纤维化。

Objective ： To detect the prophylactic effects of cureumin on rats hepatic fibrosis. Methods： The rats were divided into 6 group, normal control group, model group, positive control group, high - dose, middle - dose and low - dose curcumin group, except normal group, all other group were given CCl4 to induce liver fibrosis. When the research was over, the index of liver was detected. The activity of AST and ALT in all 6 group were detected. Morphology changes were observe by HE and Masson stain. Results： The liver index of model group was lower than that of the normal control group （ P 〈0.01 ）, and after curcuwin was applied, the liver index were higher than that of modal group （ P 〈 0.05 ）. Compared with normal control group, the activity of AST and ALT of the model group were higher significantly （ P 〈 0.01 ）, after treated with cureumin, the activity of AST and ALT were decreased significantly vs model group （ P 〈 0.05）. HE and Masson stain show that the lesion of liver in colchiein - treated group and curcumin - treated group was more mitigated than model group, and the degree of live fibrosis was also lesser （ P 〈0.05）. Conclusion： The curcumin can protect the function and morphology of liver and avoid or delay the liver injury by CCl4.