PEX基因修饰人骨髓间质干细胞体外培养鉴定的研究

In vitro culture and identification of human bone marrow mesenchymal stem cells modified by PEX gene

目的构建PEX基因真核表达载体并转染人骨髓间质干细胞(human bone marrow mesenchymal stem cells,hMSC),探讨PEX修饰hMSC的可行性。方法采用全贴壁细胞分离法培养hMSC,流式细胞技术鉴定其表面标记物;分子克隆技术构建PEX基因真核表达载体,重组质粒转染hMSC,验证真核表达载体在hMSC中的表达。结果成功培养hMSC并成功构建PEX基因真核表达载体,PEX在转染的hMSC中高表达。结论构建的PEX基因真核表达载体可用于转染hMSC,为进一步探讨其在胶质瘤治疗中的作用奠定了基础。

Objective To explore the feasibility of modifying human bone marrow mesenchymal stem cells with PEX gene （hMSCs） by the construction of the eukaryotic expression vector （EEV） of PEX gene and the transfection of EEV of PEX gene into the hMSCs. Methods Whole cell adherence method was employed to isolate and culture the hMSCs; meanwhile, flow cytometry was adopted to identify the surface marker of hMSCs. Through molecular cloning technology, EEV of PEX gene was constructed, and transfected into the hMSCs and then the expression of the EEV was verified. Results The hMSCs were isolated and the EEV of PEX gene was constructed successfully. PEX gene was highly expressed in the transfected hMSCs. Conclusion The constructed EEV of PEX gene can be used to transfect hMSCs, which lays a foundation for exploring its potential role in glioma treatment.