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脱氢表雄酮在体外对胸腺细胞的调节机制研究

Effect of dehydroepiandrosterone on thymocytes in vitro
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摘要 目的通过观察脱氢表雄酮(DHEA)对胸腺细胞凋亡及CD95/CD95配体(Fas/FasL)表达的影响,探讨在免疫系统中的作用。方法四甲基偶氮唑盐比色分析法(MTT法)观察细胞的活力,用梯度状凝胶电泳观察细胞凋亡,反转录-聚合酶链反应(RT—PCR)法检测Fas与Fas—L的表达。结果DHEA在体外降低胸腺细胞活力,促进细胞的凋亡,上调Fas/Fas—L mRNA的表达。结论DHEA能通过Fas/FasL途径诱导胸腺细胞凋亡,提示DHEA可能在一些凋亡失调相关的自身免疫性疾病的发生发展中起作用。 Objective To study the effect of dehydroepiandrosterone (DHEA) on the immune system by measuring the apoptosis and expression of Fas/FasL mRNA of thymocytes. Methods The cell viability of thymocytes was determined by MTT assay. Thymocyte apoptosis was detected by agrose gel electrophoresis. The expression of Fas and Fas-L was analyzed by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results DHEA decreased the cell viability of thymocytes and induced thymocytes apoptosis and increased the levels of mRNA of Fas/FasL. Conclusion DHEA can induce apoptosis of thymocytes through Fas/Fas-L pathway. It suggests that DHEA may play a role in certain autoimmune diseases related to apoptosis disturbance.
作者 梁军 孙凌云
出处 《中华风湿病学杂志》 CAS CSCD 北大核心 2009年第8期547-549,共3页 Chinese Journal of Rheumatology
基金 江苏省科技兴卫工程项目(RC2007002)
关键词 去氢表雄酮 胸腺 细胞凋亡 抗原 CD95 Dehydroepiandrosterone Thymus gland Apoptosis Antigens, CD95
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