Toll样受体7及9在系统性红斑狼疮患者外周血B淋巴细胞内的表达及意义

The expression and clinical significance of Toll-like receptor 7 and 9 in peripheral blood B cells of patients with systemic lupus erythematosus

目的了解Toll样受体（TLR）7及9在系统性红斑狼疮（SLE）患者外周血B淋巴细胞内的表达情况及其意义。方法流式细胞术检测50例SLE患者及30名健康人外周血B淋巴细胞内TLR7及9的表达水平。并将其与有关临床及实验室指标进行相关性分析。结果SLE患者B淋巴细胞内TLR7^＋及9^＋细胞所占比例均高于对照组。TLR7与患者红细胞沉降率（ESR）、SLE疾病活动指数（SLEDAI）呈正相关，与C3呈负相关。TLR9与患者尿蛋白、SLEDAI呈正相关。结论TLR7及9在SLE患者B淋巴细胞内表达上调，且与病情有一定的相关性。

Objective The aim of this study is to examine the expressions of Toll like receptor （TLR） 7 and TLR9 in the peripheral blood B lymphocytes of SLE patients and to analyze the correlation between TLR7/9 and clinical parameters. Methods Intracellular expression of TLR7/9 in the peripheral blood CD19^＋B lymphocytes was analyzed in 50 SLE patients and 30 healthy controls by flow cytometry. The difference of intracellular TLR7/9 expression levels in two groups was compared. Furthermore,the correlation between TLR7/9 expression and clinical parameters such as ESR, CRP, complement 3 （C3）, complement 4 （C4）, the level of serum IgG, anti-double stranded DNA antibody, anti-nuclear antibodies, SLEDAI score and urine protein excretion level, were analyzed. Results Compared with healthy subjects, the proportion of B cells expressing TLR7 and TLR9 was higher among SLE patients. Positive correlation was observed between TLR7 expression levels and clinical measurement of the SLEDAI and ESR. Negative correlation was observed between TLR7 expression levels and serum C3 levels. Positive correlation was observed between TLR9 expression levels and SLEDAI scores. Negative correlation was observed between TLR9 expression levels and serum C3 levels. Conclusion TLR7 and TLR9 expression is increased in the peripheral blood B cells of SLE patients, and correlates well with clinical parameters.