大鼠脑创伤后白细胞介素-1β和肿瘤坏死因子-αmRNA在脑组织中的表达

Expression of interleukin1β and tumor necrosis factorα mRNA in brain tissue in rats after brain injury

目的：研究大鼠实验性脑创伤后脑组织中白细胞介素１β（ＩＬ１β）及肿瘤坏死因子α（ＴＮＦα）ｍＲＮＡ表达水平的变化。方法：由液压型创伤装置致大鼠一侧脑挫伤，利用地高辛标记的寡核苷酸探针原位杂交技术观测ＩＬ１β和ＴＮＦα的基因表达。结果：鼠脑创伤后上述两种细胞因子的基因表达水平迅速增高，在伤后１小时即可达到最高水平，并持续至伤后６小时，伤后２４小时后基本恢复至正常水平。两种细胞因子在脑组织中的表达区域很类似，主要出现于创伤侧及对侧脑皮质、海马、胼胝体、脉络丛等部位。结论：脑创伤后炎性细胞因子主要由中枢神经系统内部产生。

Objective:To examine the expression of tumor necrosis factorα(TNFα) and interleukin1β(IL1β) mRNA in rat brain following experimental brain injury.Methods:A model of brain injury was induced in rats using lateral fluid percussion,and gene expression was detected in brain by in situ hybridization.Results:A marked increase in IL1β and TNFα mRNA expression was observed as early as 1 hour after trauma in injured cortex,contralateral cortex,hippocampus,corpus,callosum,choroid plexus,etc,and increased expression persisted in these regions up to 6 hours following injury.IL1β and TNFα mRNA returned to normal 24 hours after injury.Conclusions:These results indicate that following traumatic brain injury,the temporal expression of IL1β and TNFα mRNA appears to be regionally induced in specific brain regions,which may play a role in the posttraumatic pathologic or regenerative responses secondary to brain injury.