紫草酸在大鼠体内的药动学研究

Pharmacokinetics of Lithospermic Acid in Rats

目的建立紫草酸血药浓度的高效液相色谱测定方法,研究其在大鼠体内药代动学变化规律。方法大鼠静脉注射紫草酸10mg/kg后,于不同时间点采血,用HPLC测定其血药浓度,并用DAZ软件拟合,计算药代动力学各参数。结果紫草酸血药浓度的回归方程的线性关系良好,最低检测浓度为0.2μg/ml。药时曲线符合二室开放模型。结论首次建立了紫草酸血药浓度的HPLC测定方法。方法操作简单、专属性强、灵敏度高。静脉注射紫草酸,其体内过程的药时曲线符合二室开放模型,具有体内分布快、血药浓度下降迅速等特点。

Objective In order to investigate pharmacokinetics of lithospermic acid in rats, the HPLC method was established to determine the lithospermic acid in rat plasma. Methods A dose of lithospermic acid （10 mg/kg, iv） was given in rats and blood samples were collected at different time. The DAZ software was used to calculate the pharmacokinetic parameters of lithospermic acid. Results A typical equation was established to calculate the lithospermic acid in rat plasma. The lowest lithospermic acid concentration was 0. 2μg/ml. The mean plasma concentration - time curve was found to fit two compartment models. Condusion This is the first time to measure the concentration of lithospermic acid in rat plasma using the HPLC method. The developed method has been proved highly simple, sensitive and selective for studying the pharmaconetics of lithospeimic acid. The lithospermic acid concentration -time curve is a two compartment open model. The pharmacokinetics of lithospermic acid shows a rapid absorption and quick elimination precess in rats.