三种垫料丙酮提取物对小鼠肿瘤细胞株毒性作用观察

The Cytotoxic Effects of Acetone Extractions from Three Kind Beddings on Mouse Tumour Cell Lines

目的探讨实验动物垫料对小鼠细胞生长活性的影响及其可能的细胞毒性。方法体外培养的小鼠肝癌细胞Hepal-6（mouse hepatoma cell line Hepal-6，Hepal-6）、小鼠肺癌细胞（Lewis Lung carcinoma，LLC）和小鼠黑色素瘤细胞B16（Melanoma，B16）培养液中分别加入不同浓度的3种实验动物常用垫料（玉米芯、松木、杨木）丙酮提取物，采用MTT方法测定细胞生长活性，并比较3种垫料对细胞的生长毒性。结果小鼠Hepal-6细胞生长活性与3种垫料之间的线性相关系数分别为：玉米芯-0．875。松木-0．747，杨木-0．826；LLC细胞分别为：玉米芯-0．527，松木-0．812，杨木-0．865；B16细胞分别为：玉米芯-0．727，松木-0．860，杨木-0．888。松木、杨木、玉米芯垫料对小鼠Hepal-6细胞的半致死量（IC50）分别为：8．86，19．10，91．37mg／ml；对LLC细胞的IC50分别为：4．42，7．72，24．65mg／ml；对B16细胞的IC50分别为：8．86，19．10，91．37mg／ml。结论3种垫料与小鼠细胞Hepal-6、LLC、B16的生长活性之间具有显著负相关性（P〈0．001），其抑制细胞生长作用依次为：松木〉杨木〉玉米芯。

Objective To investigate the cytotoxical effects of beddings on growth activity of mouse cell lines. Methods The acetone extraction of three kind beddings（corn cob, pine wood shaving, poplar wood shaving ） were respectively added in mouse hepatoma cell line Hepal-6（Hepal-6）, the Lewis Lung carcinoma （LLC）and the Melanoma B 16（B 16） with different concentration. MTT method was used to detect the cell grown activity and compare the cytotoxical effects of different beddings（pine wood shaving, poplar wood shaving and corn cob）. Results The acetone extraction of three kinds of bedding provided with inhibition effects on three cell lines. The coefficients on Hepal-6 in linear regression models are： corn cob -0.875, pine wood shaving -0.747, poplar wood shaving -0.826. The coefficients on LLC are： corn cob -0.527, pine wood shaving -0.812, poplar wood shaving -0.865. The coefficients on B16 are： corn cob -0.727, pine wood shaving -0.860, poplar wood shaving -0.888. The 50% concentration of inhibition （IC50） of beddings to Hepal-6 are： corn cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37mg/ml. The IC50 of beddings to LLC are： corn cob 4.42 mg/ml, pine wood shaving 7.72 mg/ml, poplar wood shaving 24.5 mg/ml. The IC50 of beddings to B16 are： corn cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37 mg/ml. Conclusion There are evident negative relativity between the three beddings and Hepal-6, LLC, B 16 cell grown activity （P〈0.001）. Cytotoxic role in cell growth inhibition is pine wood shaving〉 poplar wood shaving〉 com cob.