扩展青霉拮抗放线菌CCTCC M207210液体培养条件的优化

Optimization of conditions for liquid cultivation of the antagonistic actinomycete CCTCC M207210 to Penicillium expansum

【目的】优化扩展青霉拮抗放线菌CCTCC M207210的液体发酵条件,为其大量生产提供技术支撑。【方法】采用单因素试验和Box-Benhnken试验设计,研究培养温度、初始pH、装液量、接种量对放线菌CCTCCM207210菌体干质量的影响,通过响应面分析优化了各因素综合影响下的培养条件。【结果】放线菌CCTCCM207210的最优培养条件为:接种量135 mL/L,初始pH 7.4,摇床转速180 r/min,培养温度27.6℃,250 mL摇瓶装液量125 mL。在此条件下培养72 h,菌体干质量的预测值为6.27 g/L,验证值为6.33 g/L。【结论】在最佳液体培养条件下,CCTCC M207210最大菌体干质量为6.27 g/L,是优化前的2倍。

[Objective] The paper aimed at optimizing the conditions for liquid cultivation of the antagonistic actinomycete CCTCC M207210 to Penicillium expansurn thus providing information for scaling up the cultivation. [Method] Single-factor design and Box-Benhnken design were used to select the important factors and their level region. Response Surface Analysis was used to optimize the conditions with complex effect among various factors. [Result] The optimal conditions were obtained at inoeulums＇ amount of 135 mL/L,initial pH 7.4,rotation speed 180 r/min,27.6 ℃ ,and medium volume of 125 mL in 250 mL flask. [Conclusion] Under the optimal conditions,the dried cell weight of strain CCTCC M207210 reached 6.27 g/L,2 times to the original one.