生长抑制激素和生长激素对急性坏死性胰腺炎肝损伤的保护作用

Somastostatin and growth hormone in preventing liver damage due to acute necrotizing pancereatitis

目的探讨生长抑制激素（简称生长抑素）和生长激素对急性坏死性胰腺炎（ＡＮＰ）肝损伤的保护作用。方法３５％牛磺胆酸逆行胰胆管注射，建立ＡＮＰ大鼠模型。观察炎性介质［内毒素、淀粉酶、肿瘤坏死因子α（ＴＮＦα）］的变化并用逆转录多聚酶链反应（ＲＴＰＣＲ）分析ＴＮＦαｍＲＮＡ转录水平的表达，及生长抑素和生长激素的治疗作用。结果ＡＮＰ可以诱导炎性介质明显升高，并与肝损伤的严重程度呈正相关，而且肝的ＴＮＦαｍＲＮＡ也呈高表达。生长抑素和生长激素能抑制ＡＮＰ的炎性介质过度释放及肝ＴＮＦαｍＲＮＡ的转录水平的高表达，减轻胰腺和肝的损伤程度。结论炎性介质参与了ＡＮＰ对肝脏的损伤。

Objective To study the role of somastostatin and growth hormone in preventing liver damage due to ANP. Methods The roles of inflammatory mediators (Endotoxin, Amylase, TNFα) were investigated in ANP model by retrograde injection of 3.5% sodium taurocholate 2.5 ml/kg into the pancreatic duct, and TNFα mRNA in the liver after ANP was observed by reverse transcriptasepolymerase chain reaction. Besides, the effects of somastostain and growth hormone were also observed. Results ANP caused remarkable elevation of those inflammatory mediators, being positively correlated with the development of pancreas and liver damage. Somastostain and growth hormone inhibited the inflammatory mediates and TNFα mRNA overexpression and reduced the damage to the pancreas and liver. Conclusions TNFα plays an important role in ANP progression, and somastostatin and growth hormone may prevent the development and progression of liver damage due to ANP.