PPAR-γ、survivin在直肠癌中的表达及其临床意义

Expression and clinical significance of PPAR-γ and survivin in rectal cancer

目的探讨过氧化物酶体增殖因子活化受体γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)、存活素(survivin)在正常直肠组织、腺瘤和直肠癌中的表达情况与临床病理关系,并评价其在直肠癌预后判断中的价值。方法利用组织芯片和免疫组织化学技术,检测正常直肠、腺瘤组织标本各16例及直肠癌80例中PPAR-γ、survivin基因蛋白表达,并回顾性分析PPAR-γ、survivin与各临床病理指标及预后间关系。结果80例直肠癌标本中PPAR-γ、survivin表达阳性明显高于腺瘤及正常直肠组织(P<0.05)。直肠癌患者不同年龄、性别、组织学类型的PPAR-γ、survivin表达程度差异无统计学意义(P>0.05);不同分化程度、病理分期、淋巴转移、3年生存率的PPAR-γ表达程度差异有统计学意义(P<0.05);直肠癌中survivin与PPAR-γ基因表达间关系密切。结论PPAR-γ、survivin基因表达异常与直肠癌的发生密切相关,检测PPAR-γ及survivin蛋白对诊断直肠腺癌、判断其恶性程度及预后均有一定参考价值。

[Objective] To investigate the relationship between expression of peroxisome proliferator activated receptor-γ （PPAR-γ）, survivin and the elinicopathologic parameters in rectal carcinoma, and evaluate their significances in pradicating the prognosis. [Methods] Tissue microarray and immunohistochemistry SP were used to detect the expression of PPAR-γ gene and survivin in 16 cases of the normal rectal tissue, 16 cases of rectal adenoma and 80 cases of the rectal carcinoma. The relationship between the expression of PPAR-＇y, survivin and the clinicopathologic parameters, prognosis were retrospectively analyzed. [Results] The positive rates of PPAR-γ and survivin expression in adenocarcinoma were higher than those in matched normal tissues （P〈0.05）. Clinicopathological analysis of patients showed that the increase of PPAR-γ and survivin expression were not associated with age, sex and histological classification （P 〉0.05）, but were associated with differentiation grades, pathological stages, lymph nodes metastases and 3-year survival rate （P 〈0.05）. The expressions of PPAR-3＇ and survivin were positively correlated （P 〈0.05）. [Conclusions] PPAR-γ and survivin gene may play a role in the oncogenesis and progression of rectal carcinoma. Detection of PPAR-γ and survivin are valuable for diagnosing rectal carcinoma, and evaluating malignancy extent and prognosis.