哮喘患儿外周血IL-2,IL-10与NO水平及其关系研究

STUDY ON THE INTERLEUKIN2,INTERLEUKIN10 AND NITRIC OXIDE LEVELS IN ASTHMATIC CHILDREN AND THEIR RELATIONSHIP

采用双抗夹心ＥＬＩＳＡ技术检测了２８例哮喘患儿血浆及外周血单个核细胞（ＰＢＭＣ）在植物血凝素（ＰＨＡ）刺激下产生ＩＬ－１０水平，同时检测了ＮＯ和ＩＬ－２水平。结果：①急性发作期哮喘患儿血浆及ＰＢＭＣ在ＰＨＡ刺激下产生ＩＬ－１０水平均比正常儿童显著降低（Ｐ＜０．０１），在哮喘缓解期虽均有所恢复，但仍低于正常儿童（Ｐ＜０．０５）；②ＮＯ水平在急性发作期患儿明显升高（Ｐ＜０．０５），而在缓解期恢复正常（Ｐ＞０．０５）；③急性发作期哮喘患儿在ＰＨＡ刺激下ＰＢＭＣ产生ＩＬ－２水平明显升高（Ｐ＜０．０１），但在缓解期恢复正常（Ｐ＞０．０５）；④急性发作期哮喘患儿在ＰＨＡ刺激下ＰＢＭＣ产生ＩＬ－１０水平与ＩＬ－２呈显著负相关（ｒ＝－０．５０４，Ｐ＜０．０１），而与血浆ＮＯ水平相关不显著（ｒ＝－０．３１９，Ｐ＞０．０５）。提示：哮喘发作时ＩＬ－１０分泌及释放减少，ＮＯ和ＩＬ－２水平升高，导致或加重气道慢性炎症反应，引起气道高反应性而诱发或加重哮喘。

We used double antibody sandwich ELISA for the quantitative determination of plasma IL10 level and the production of IL2, 10 by PHAactivated peripheral blood mononuclear cells (PBMC) level in 28 asthmatic children and 20 matched normal children. At the same time, the plasma nitrite and nitrate(NO2/NO3) level was determined. Results showed that asthmatic children in acute attack stage(pretherapy) had decreased amounts of the plasma IL10 and the production of IL10 by PHAactivated PBMC compared with normal control subjects (7.21±2.22,11.16±2.88;22.16±5.58,64.11±10.44pg/ml, respectively, both of the P<0.01), while in restoration stage (posttherapy) which had restored in some degree, but still lower than the normal levels(9.25±1.62,56.84±10.43,respectively, P<0.05). Asthmatic children in acute attack stage had increased amounts of the plasma NO2/NO3 and the production of IL2 by PHAactivated PBMC compared with normal control subjects (NO2/NO3:32.67±4.51,29.64±2.93μmol/L;IL2:21357±5978,16152±3411cpm, respectively, P<0.05,0.01 respectively),while in restoration stage which had decreased amounts of the plasma NO2/NO-3 and the production of IL2 by PHAactivated PBMC to normal levels (NO2/NO3:30.18±3.09μmol/L;17068±3514cpm,respectively, both of the P>0.05). Linear correlation analysis showed obvious correlation between the production of IL10 and IL2 by PHAactivated PBMC. The results suggest that there was decreased release and secretion of IL10, and increased production of IL2 and NO in asthmatic children, which could cause or increase airway hyperresponsiveness in some degree. They might play important roles in immunological mechanisms of asthma.