小鼠 Lewis 肺癌细胞株L3-8的建立及生物学特征

Establishment of C57BL Mice Lewis Lung Carcinoma Cell Line(L3-8)and its Cellbiology Character

为了得到高转移率的小鼠肺癌体外传代细胞株，将Ｌｅｗｉｓ肺癌实体瘤组织在体外进行１４个月的传代培养，获得了Ｌ３－８细胞株。对该细胞生物学特征的研究结果表明，第３９代和第６５代细胞的倍增时间分别为２３．５ｈ和２６．２ｈ；第８０代细胞的平皿和软琼脂集落形成率分别为３０．７％和２１．２％；在Ｃ５７ＢＬ小鼠体内的成瘤率为１００．０％，肺转移率为８３．３％，平均转移灶２．９个。此细胞株较原体内传代株具有更高的转移率，为肿瘤细胞生物学。

In order to acquire a cell line with high metastases rate,the lewis lung cancinoma tissue of C57BL mice was cultured in vitro for 14 months,and a cell line designated as L3-8 was derived from it.The results of cellbiological studies:The doubling time of the 39th and 65th passage cells was 23.5h and 26.2h respectively.Plating effeciency and soft agar colonogenic effeciency was 30.7% and 21.2% respectively.Tumor was taken when 3×106～4×106 cells(L3-8) were transplanted into the C57BL mice and 83.3% of the mice had lung metastases.The average nodules of metastases was 2.9.This cell line may be a useful tool in the studies of oncologyical cellbiology,immunology and pharmacology.