肺癌病人外周血T淋巴细胞亚群、NK活性和IL-2水平的研究

The Study on T-lymphocyte Subcategories, NK cell Activity and IL-2 Level in Patients with Lung Cancer

目的探讨肺癌不同阶段机体细胞免疫功能状态。方法选择肺癌病人27例（Ⅱ期以下13例，N期以上14例），健康对照14例。取外周静脉血提取单个核细胞（PBMC），单抗致敏红细胞花环法测定T淋巴细胞亚群；以K562细胞为靶细胞，3H－TdR法测定NK活性；用PHA刺激PBMC产生IL－2，IL－2依赖细胞3H－TdR掺入法检测。结果Ⅱ期以下肺癌组CD。百分比（％）、NK活性（u）和IL－2水平（u）分别为：32．92±10．85％；20．56±3．88u；10．62±2．64u。对照组分别为42．70±2．81％；28．50±2．29；19．39±3．13。二组三项指标对比P值均＜0．05．N期以上肺癌组三项指标分别为28．26±8．26％；16．24＋5．66u；9．59±2．70u，与对照组比较P值均＜0．01。结论肺癌病人的细胞免疫功能明显降低，随病情进展，纵膈及远隔器官转移的出现，免疫功能进一步下降。提示肺癌病人采用放疗或化疗的同时，应辅以免疫治疗。

Objective: To study the cyto-immllnity condition of patients with lung cancer in dif-ferent stages. Method:Twenty-seven lung cancer patients (13 cases before stageⅡand 14 cases afterstage Ⅲ) served as subjects and 14 health persons as control. Peripheral blood mononuclear cells(PBMC) were collected from vein blood. T-lymphocyte subcategories were measured with method of al-lergical erythrocyte garland by monocolonal antibody. NK activity was measured with 3H-TdR methodbased on K562 cells which served as target cells. IL-2 produced from PHA-stimulated PBMC was mea- measured with CTLL 3H-TdR permeating method. Result: The percent of CD4 (%), NK activity (u) andIL-2 level (u) in patients with lung cancer before stage Ⅱ were 32. 92±10. 85 %, 20. 56±3. 88u and10. 62±2. 64u, respectively. The results from control group were 42. 70±2. 81 %, 28. 50±2. 29u and19. 39±3. 13u, (P<0. 01), respectively. The three indexes in patients with lung cancer after stage Ⅲwere 28. 26±8. 26 %, 16. 24±5. 66u and 9. 59± 2. 70u, (P<0. 01), respectively. Conclusion: The cyto-immunity functions in patients with lung cancer decreased significantly compared with those from con-trol. The functions were getting worse with the occurrence of tumor metastasis. Therefore, it is valu- able to increase their immunity function when radiotherapy and chemical pharrnacotherapy were admin-istered to patients with lung cancer.[