胃癌组织bcl-2蛋白表达和DCC基因的杂合性缺失

Expression of bcl-2 Protein and loss of Heterozygosityat DCC Gene in Gastric Cancer

为了研究ｂｃｌ２蛋白表达和ＤＣＣ基因的杂合性缺失（ＬＯＨ）在胃癌发生中的作用，采用免疫组织化学技术和ＰＣＲ方法检测了胃癌组织ｂｃｌ２蛋白的表达和ＤＣＣ基因的杂合性丢失（ＬＯＨ）。结果发现，胃癌（信息个体）ＬＯＨ发生率为５４．５％（１８／３３），ｂｃｌ２蛋白的表达阳性率为６０．０％（３０／５０），Ⅲ、Ⅳ期胃癌ＬＯＨ发生率（８３．３％，１５／１８）显著高于Ⅰ、Ⅱ期胃癌（２０．０％，３／１５），Ｐ＜０．０１。ｂｃｌ２蛋白的表达和ＤＣＣ基因的ＬＯＨ与胃癌大小、分化程度、淋巴结转移、浆膜浸润、临床分期及Ｌａｕｒｅｎ′ｓ分型无显著相关。结果提示ｂｃｌ２蛋白的高表达参与了胃癌的发生，ＤＣＣ基因的ＬＯＨ与胃癌的进展有关。

In order to investigate the roles of expression of bcl-2 protein and LOH at DCC gene in the carcinogenesis of gastric cancer,the expression of bcl-2 protein and LOH at DCC gene were detected by immunohistochemical technique and PCR method.LOH was detected in 18/33(54.5%) of gastric cancers,bcl-2 protein was detected in 30/50 (60.0%).The frequence of LOH in stage Ⅲ～Ⅳ gastric cancer was significantly higher than that in Ⅰ～Ⅱ stage (P<0.01).The expression of bcl2 protein was not associated with LOH at DCC gene,tumor size,differentiation,lymphnode metastasis,serosal invasion,clinical stage and Lauren′s type.The results suggest that high expression of bcl-2 protein is involved in the development of gastric cancer and LOH at DCC gene is involved in its progression.Their carcinogenetic mechanism milght be different.