胃癌组织中增殖细胞核抗原、P53、K-ras及Bcl-2蛋白的表达

EXPRESSION OF PCNA AND P53, K ras, Bcl 2 PROTEINS IN GASTRIC CANCER

为了解胃癌组织中多种基因蛋白异常表达及意义，应用免疫组化技术检测５１例手术切除胃癌组织标本中增殖细胞核抗原（ＰＣＮＡ）及Ｐ５３、Ｋ－ｒａｓ、Ｂｃｌ－２蛋白的表达。结果胃癌组织中ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）为５１．２±２０．５，Ｐ５３，Ｋ－ｒａｓ，Ｂｃｌ－２蛋白表达率分别３７．３％，１７．６％和６８．６％，均显著高于正常胃粘膜（Ｐ＜０．０５）。ＰＣＮＡＬＩ与肿瘤大小、淋巴结转移及临床分期密切相关（Ｐ＜０．０５）。肠型胃癌中Ｋ－ｒａｓ、Ｂｃｌ－２蛋白表达率分别为４３．８％和９３．８％，均显著高于胃型胃癌的６．０％（Ｐ＜０．０１），５４．５％（Ｐ＜０．０５），而ＰＣＮＡＬＩ及Ｐ５３蛋白表达率两型胃癌无显著差别。提示ＰＣＮＡ、Ｐ５３、Ｋ－ｒａｓ、Ｂｃｌ－２蛋白异常表达与胃癌的发生发展有关。ＰＣＮＡＬＩ可能对判断胃癌的预后有一定价值。Ｋ－ｒａｓ。

In order to investigate the role of multiple genetic expression in the development and progress of gastric carcinoma(GC), the expressions of PCNA, P53, K ras, Bcl 2 proteins were detected in 51 surgically resected GC specimens with immunohistochemical technique. It was found that PCNALI in GC was 51 2±20 5 and expressions of P53, K ras, Bcl 2 proteins were 37 3%, 17 6% and 68 6%, respectively. PCNALI was positively correlated with tumor size, lymphonode metastesis and clinical stages( P <0 05). The expression rates of K ras(43 8%) and Bcl 2(93 8%)in intestinal type GC were significantly higher than those of diffuse type of GC(6 0%, 54 5%) ( P <0 01, P <0 05), however no significant difference of P53 protein expression and PCNALI was found between the two type GC. These findings suggested that the abnormal expressions of PCNA, P53,K ras,Bcl 2 were corelated with the development and progress of GC and PCNALI may be of some value for predicting prognosis of GC. K ras, Bcl 2 may be two susceptable target genes of GC.