幽门螺杆菌清除前后胃粘膜上皮细胞凋亡的变化

Effect of Helicobacter Pylori on Gastric Epithelial Apoptosis

研究幽门螺杆菌（Hp）感染对胃粘膜上皮细胞凋亡的影响及其在胃癌发生中的意义。采用脱氧核糖核酸末端转移酶介导的缺口末端标记（TUNEL）技术对Hp阴性患者、Hp阳性患者Hp清除前后胃粘膜上皮中凋亡细胞的分布和密度进行原位观察和比较。结果：发现Hp阳性患者胃粘膜上皮细胞凋亡指数（16.01％），明显高于Hp阴性患者（3．52％）（P＜0．01）；胃粘膜细胞凋亡指数与Hp感染程度明显相关（P＜0．05）；Hp清除后胃粘膜上皮细胞凋亡指数（4.19％）较治疗前明显降低（P＜0．01），而持续阳性者凋亡指数无明显降低。提示：Hp感染可诱导胃粘膜上皮细胞凋亡，这可能是Hp参与胃癌发生的重要机制之一。

To study th effect of Hp on gastric epithelial apoptosis. Methods: Using terminal deosynuclotidyl transferase-mediated dUTP nick end labelling(TUNEL) technique,we observed the apoptotic cells in situ in Hp negative gastric mucosa,Hp positive gastric mucosa before and after Hp clearance. Results:The apoptotic index (16. 01%) of gastric epithelial in the patients infectd with Hp was significantly higher than that of Hp negative gastric epithelial (with apoptotic index 3. 52%) (P＜0.01). After clearance of Hp with triple therapy,the apoptotic index fell from 16. 07% to 4.19% (P＜0. 01 ). In the persisting Hp infected patients,the apoptotic index was not statistically decreased. Conclusion:Hp infection may induce the apoptosis of gastric epithelial cells in vivo. Hp infection may be one of the risk factors of gastric carcinogenesis.