CD44V6的表达对大肠癌细胞缝隙连接细胞间通讯的影响

EXPRESSION OF CD44V6 AND ITS EFFECTS ON GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN HUMAN COLORECTAL CARCINOMA CELLS

应用原位分子杂交、免疫组化、免疫荧光定量分析的方法研究ＣＤ４４Ｖ６在人大肠癌细胞中的表达，应用激光漂白后荧光恢复技术研究ＣＤ４４Ｖ６对大肠癌细胞的缝隙连接细胞间通讯的影响。结果表明，ＣＤ４４Ｖ６在ＨＴ２９、Ｌｏｖｏ细胞中均有表达，不管在ｍＲＮＡ水平还是在蛋白质水平，高转移能力的Ｌｏｖｏ细胞表达ＣＤ４４Ｖ６均高于低转移能力的ＨＴ２９细胞。将ＨＴ２９、Ｌｏｖｏ细胞用ＣＤ４４Ｖ６单抗封闭则发现ＣＤ４４Ｖ６不能使ＨＴ２９、Ｌｏｖｏ细胞的缝隙连接细胞间通讯发生明显改变。说明ＣＤ４４Ｖ４６的表达与大肠癌细胞的转移潜能有关。

Expression of CD44V6 variant in human colorectal carcinoma HT29 and Lovo cell lines was investigated by in situ hybridization, immunohistochemistry and quantitative immunofluorescenece technique at mRNA and protein level. The effect of CD44V6 variants on gap junctional intercellular communication (GJIC) was studied by fluorescence recovery after photobleaching (FRAP). The result showed that both of HT29 and Lovo cell lines were well expressed in the CD44V6 mRNA and their proteins, but Lovo cells expressed more highly than HT29 cells both at mRNA and protein levels. Moreover, the expressions of CD44V6 were different among Lovo cell lines, part of which expressed more.The expression of CD44V6 variant was related to metastatic potential of human colorectal carcinoma cells,but CD44V6 could not affect the GJIC of HCC cells.