肝癌和肝硬化患者庚型肝炎病毒RNA及NS3区cDNA酶切分析

Detection of hepatitis G virus RNA and restrict endonulease analysis of NS3 gene cDNA in sera of patients with hepatocellular carcinoma and liver cirrhosis

目的探讨肝癌及肝硬化患者中ＨＧＶ的感染状态及ＮＳ３区部分基因的酶切分析。采用ＲＴＰＣＲ技术检测ＨＧＶＲＮＡ，酶切分析比较中国株与美国ＧＢＶ－Ｃ株两种基因型的酶切位点。结果８１例肝癌样品中９例ＨＧＶＲＮＡ阳性（１１．１１％），２８例肝硬化中，３例ＨＧＶＲＮＡ阳性（１０．７％）。酶切位点分析结果表明，中国ＨＧＶ感染患者在ＧＢＶ－Ｃ４３５１位存在ＴＳＰ５０９Ｉ（ＡＡ↓ＴＴ）切点，美国和日本发表的１１株ＧＢＶ－Ｃ和１株ＨＧＶ中仅２株存在此切点。结论酶切分析结果提示ＧＢＶ－Ｃ株与中国ＨＧＶ感染株。

To detect the presence of hepatitis G virus infection in patients with hepatocellular carcinoma and liver cirrhosis and analyze the NS3 gene with restrict endounclease.RT PCr technique was used to detect HGV RNA.Positive PCR products were digested with restrict endonuclease to find the existence of certain recognization site.Results:9 samples(11.1%) were HGV RNA positive in 81 patints with hepatocellular carcinoma,and 3(10.7%) were positive in 28 patients with liver cirrhosis.When digested with restrict endonuclease,all Chinese isolates had TSP 509I (AA↓TT) recognization site in nt 4351 (GBVC position),however,only 2 of 12 American and Japanese isolates (11 GBVC isolates and 1 HGV isolate)had this site.Conclusion:The result of restrict endonuclease analysis demonstrated that Chinese HGv isolate and GBVC isolate may belong to different genotype.