荷叶总生物碱脂质体的制备及质量评价

Preparing the Liposomes of Total Alkaloids of Lotus Leaf and Carrying on the Quality Appraisal

目的:制备荷叶总生物碱脂质体,并进行质量评价。方法:采用逆相蒸发法制备荷叶总生物碱,以阳离子交换树脂法分离脂质体,于164nm处测定吸光度,计算包封率;考察油水比、卵磷脂及药物的加入量对包封率的影响。结果:实验表明,油水比为4∶1,卵磷脂量为250mg时,所得脂质体呈球型或椭圆型,包封率为84.9%。结论:本法制备荷叶总生物碱脂质体包封率较高。

Objective ：To prepare the Liposomes of total alkaloids of lotus leaf, and carry on the quality appraisal. Method：Reverse phase evaporation was adopted to prepare the Leptosomes of total alkaloids of lotus leaf, the Liposomes was separated by the cation exchange resin,and the absorbance was measured at 164nm, and the encapsulation efficiency was calculated ,the impact of oil -water radio, Lecithin and the addition of drug towards encapsulation efficiency was inspected. Results：The experiment indicats,while the oil-water radio is 4：1 ,the quantity of soybean lecithin is 250mg, Liposomes is roughly spherical or elliptic, and the encapsulation efficiency is 84.9%. Conclusion：The encapsulation efficiency of Preparing the Liposomes of total alkaloids of lotus leaf by this method is higher.