保肺定喘汤对野百合碱诱发肺血管重构大鼠VEGF表达的影响

Effect of Baofei Dingchuan Tang on the Expression of Vascular Endothelial Growth Factor in Pulmonary Vascular Remodeling Rats Induced by Monocrotaline

目的：观察保肺定喘汤对肺动脉高压肺血管重构大鼠VEGF表达的影响。方法：60只SD大鼠随机分为正常对照组、模型组、保肺定喘汤高、中、低剂量组和硝苯地平组（n=10）。采用野百合碱（60mg/kg）腹腔注射建立肺动脉高压肺血管重构大鼠模型,造模后连续用药28天后处死大鼠,ELISA法测定血中VEGF水平,RT-PCR测定肺组织VEGF mRNA含量,右下肺行HE及免疫组化染色观察肺小动脉病理改变及VEGF表达。结果：模型组肺小动脉管壁变厚、管腔变小,血清VEGF、肺组织VEGF mRNA以及肺小动脉VEGF表达均明显增加（P均〈0.01）,各治疗组与模型组比较肺小动脉管壁变薄,管腔增大,VEGF表达水平均有不同程度下降（P〈0.01-0.05）。结论：VEGF参与肺血管重构过程,保肺定喘汤可以减轻肺血管重构,其机理可能与其调节VEGF在血清、肺组织及肺血管表达相关。

Objective：To observe the effects of Baofei Dingchuan Tang on expression of vascular endothelial growth factor （VEGF） in pulmonary vascular remodeling model of rats. Methods ：Sixty male Sprague-Dawlcy rats were randomly divided into normal control group, model group, nifedipine therapy group, high dose, middle dose and low dose Bao Fei Ding Chuan Tang therapy group（ n = 10 for each group）. Rats were given a single dose of monocrotaline（60 mg/kg） to induce the model of pulmonary vascular remodeling,and treated with gastric infusion of normal saline,nifedipine and different dose of Bao Fei Ding Chuan Tang respectively for 28 days. The levels of VEGF in serum and VEGF mRNA in lung were measured. The light microscope and image analysis were used in analyzing HE and immunohistochemistry staining of VEGF. Results：The wall thickness increased and lumens area decreased in model group. Also the levels of VEGF in serum, VEGF mRNA in lung and expression of VEGF in micrevascular pulmonary artery increased obviously. （ P 〈 0. 01, vs. normal control group）. Each therapy group mitigated pulmonary vascular remodeling and the expression of VEGF decreased obviously. （ P 〈 0. 01 - 0. 05, vs. model group） Conclusion： VEGF plays an important role in the process of pulmonary vascular remodeling. Baofei Dingchuan Tang might reduce the remodeling of pulmonary vascular by adjusting the expression VEGF in serum,lung and microvascular pulmonary artery.