益气活血方对球囊损伤大鼠颈总动脉后P_(27)表达的诱导

Effect of Yiqihuoxue Prescriptions on Expression of P27 After Carotid Artery Balloon Injury in Rats

目的:观察损伤血管细胞周期素依赖激酶抑制蛋白P27的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉内膜平滑肌细胞增殖的干预机制。方法:随机将60只雄性SD大鼠分为模型组,阿司匹林组,益气活血方大、中、小剂量组。球囊损伤大鼠颈总动脉前给予高脂饲料喂养30天,造成高脂血症;然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉管腔的狭窄。手术前1天开始给药,每组动物于术后(14、28天)处死,取损伤颈总动脉,常规固定、包埋、切片,用免疫组织化学SABC法检测P27的表达。结果:各组P27均有表达,益气活血方大剂量组表达明显增多,用益气活血方干预后14天和28天P27表达量与模型组比较有显著性差异(P<0.05)。结论:益气活血方对球囊损伤后动脉血管内膜VSMCs增生有明显的抑制作用,其机制可能与上调P27的表达,抑制血管内膜VSMCs的细胞分裂周期进程有关。

Objective：To investigate the effect of yiqihuoxue prescriptions on expression of Pz7 after carotid artery vascular injury in rats. Methods：Rats（ n =60）were randomly divided into 5 groups： the model group;the aspirin group;the low-dose Yiqihuoxue group,the mid-dose Yiqihuoxue group and the high-dose Yiqihuoxue group. All of the rats fed up with hypereholesterolemie diet for 30 days,then the 2. Of balloon catheter injury was perfomed through the right femoral artery into the left common carotid artery of rats,the balloon was inflated and deflated 3 times. The rats was given drugs into stomach from 1 day before injury and to 14days and 28days after injury. Around 6 rats from each group were euthanized by exsanguinafion at appropriate time point. The injuried left contain carotid arteries were carefully removed and harvested for SABC staining. The expression of P27 was observed by immunohistochemical technique. Results：The expression of P27 at the 14th day after injury was minimum, and increased obviously when given Huoxueyiqi prescriptions,compared with the model group （ P 〈 0.135 ）. Conclusion： Huoxueyiqi prescriptions could effectively decrease intimal VSMCs proliferation after balloon injury,which maybe associated with inhibiring the proliferation of VSMCs through increasing the expression of P27.