脑力苏胶囊对慢性缺血VD大鼠行为学及低氧诱导因子表达的影响

Effects of NLS Capsule on Behavior And the Expression of Hypoxia Inducible Factor-la in the Hippocampus of Vascular Dementia Rats Induced by Chronic Cerebral Ischemia

目的:研究脑力苏胶囊改善VD大鼠认知障碍的作用机制。方法:根据Pappas BA方法,采用双侧颈总动脉结扎法制作VD大鼠模型,用Moriss水迷宫检测VD大鼠学习、记忆改善状况。通过免疫组化方法检测大鼠海马(CA1区)缺氧诱导因子(HIF-1α)阳性细胞数。结果:脑力苏高、低剂量灌胃治疗后大鼠学习记忆明显改善(P<0.01),脑力苏治疗组HIF-1α表达较假手术组明显增加,较模型组明显降低。结论:脑力苏可提高缺血脑组织细胞内氧浓度,改善学习记忆功能,对VD具有确切的保护作用。

Objective ：To study the action mechanism of NaoLiSu（NLS） capsule to improve the cognitive impairment of Vascular Dementia （VD） rats. Methods：According to Pappas BA＇s method, replicated VD rats model by blocking bilateral common carotid artery permanently, observing the expression of HIF-1α positive ceils in virtue of the immunohistochemistry. Results ：The learning and memory abilities of rats significantly improved after treatment of high-and low-dose （P 〈 0. 01 ）. Comparing with sham-operated group, the expression of positive cells of HIF-1 a of high-dose group increase significantly and lower than the model group. Conclusion：High dosage NLS capsule can improve the learning and memory ability significantly, NLS can increase the tolerance of neuroeyte for hypoxia,having definite protective effect.