海洋胶原肽预防SAMP8小鼠学习记忆功能下降的实验研究

Preventive Effect of Marine Collagen Peptide on Learning and Memory Impairment in SAMP8 Mice

为探讨海洋胶原肽(MCP)对痴呆性学习记忆下降的影响及作用机制,选用6月龄SAMP8小鼠为模型,分别喂饲含0、0.225%、0.450%和1.350%MCP的特殊加工饲料4个月,之后进行Morris水迷宫检测以观察小鼠的空间学习记忆能力、Nissl染色观察海马神经元的状况,同时检测海马区脑源性神经营养因子(BDNF)的表达状况。结果表明,0.44%和1.32%MCP干预可明显预防SAMP8小鼠学习记忆功能的下降;防止神经元过度丢失,促进脑源性神经营养因子的表达可能是其重要的作用机制。结果显示,海洋胶原肽具有抑制学习记忆下降的功能,可成为抗衰老相关功能食品的新选择。

To explore the preventive effect and possible mechanisms of marine collagen peptide （MCP） on AD- related memory impairment, SAMP8 mice aged 6 months were divided into control group and three MCP treatment groups. The mice were fed with chow diet, 0. 225, 0. 450 or 1. 350% （wt/wt） MCP respectively for 4 months. The Morris water maze was used to check the spatial learning and memory ability. The morphological changes of hippocam- pal tissue were estimated by nissl stain. Immunoblot analysis was used to measure the expression of brain-derived neurotrophic factor （BDNF）. The results showed that spatial memory ability in 0. 450 and 1. 350% MCP-treated mice were significantly enhanced compared to SAMP8 control mice. The reduced apoptotic neurons and up-regulated expression of BDNF were involved in the mechanism of preventive effect of MCP on AD-related memory loss. These findings suggest that MCP could he a candidate for functional food to relieve memory deficits associated with aging.