丁酸钠对 Bel-7402 人肝癌细胞几种酶活性的影响

Effects of Sodium Butyrate to the Enzyme Activity inBel-7402 Human Hepatocarcinoma Cells

为了探讨丁酸钠对人肝癌细胞各种酶活性的影响，采用丁酸钠处理Ｂｅｌ－７４０２人肝癌细胞，测定其γ－谷氨酰转肽酶（γ－ＧＴ）、酪氨酸－α－酮戊二酸转氨酶（ＴＡＴ）、醛缩酶（ＡＬＤ）的比活力和细胞增殖速度。结果显示，２．５ｍｍｏｌ／Ｌ和５．０ｍｍｏｌ／Ｌ的丁酸钠能明显抑制人肝癌细胞增殖，并使肝癌标志酶γ－ＧＴ活性及反映肝细胞恶变或损伤的ＡＬＤ活性明显降低（Ｐ＜０．０１，Ｐ＜０．０５），而使反映肝细胞分化的ＴＡＴ活性明显升高（Ｐ＜０．０１，Ｐ＜０．０５）。结果表明，丁酸钠能引起人肝癌细胞γ－ＧＴ、ＴＡＴ、ＡＬＤ明显改变。

After SB treatment,the proliferation rates and specific activities of γ-glutamyl transpeptidase(γ-GT),tyrosine-α-ketoglutarate aminotransferase(TAT) and aldolase(ALD) were detemined.The results showed that after treating with 2.5 mmol/L and 5 mmol/L SB,the cell proliferations were inhibited significantly,the specific activity of γ-GT,the liver cancer mark enzyme,was significantly decreased(P<0.05),and the specific activity of ALD which reflected liver cell cancerating and damaging was significantly decreased too(P<0.05),but the specific activity of TAT which reflected liver cell differentiating was significantly increased(P<0.05).These results indicated that SB could induce significantly varied activity of γ-GT,TAT and ALD,and these enzymes can be considered as index of differentiation of the Bel-7402 cells.