胆囊腺癌p21、p53表达及细菌L型检测的意义

Detection and Significance of the Bacterial Lform p21 and p53 in Adenocarcinoma of the Gallbladder

应用免疫组化（ＳＰ法）和革兰氏染色技术，对３６例胆囊腺癌和３０例慢性胆囊炎进行ｐ２１、ｐ５３蛋白及细菌Ｌ型检测，同时对Ｌ型阳性和阴性组织的ｐ２１、ｐ５３表达进行对比分析。结果发现，腺癌和慢性炎的革兰染色Ｌ型检出率分别为８０．６％和７６．７％，与Ｌ型抗原检出率均无显著性差异（Ｐ＞０．０５）。腺癌组的ｐ２１、ｐ５３表达率明显高于炎症组（Ｐ＜０．００５），腺癌中Ｌ型阳性组的ｐ２１、ｐ５３表达率也高于其Ｌ型阴性组（Ｐ＜０．０５）。表明Ｌ型感染与胆囊腺癌、慢性炎及ｐ２１、ｐ５３过表达有关，ｐ２１、ｐ５３过表达与胆囊腺癌也有关。提示Ｌ型参与了ｒａｓ、ｐ５３基因突变，并可能通过致基因突变而成为胆囊腺癌发生的危险因素之一。

36 cases with adenocarcinoma of the gallbladder (ACG) and 30 cases of chronic gallbladditis (CGS) associated with bacterial Lform were examined by Gram’s staining and immunohistochemistry.It showed that the positive rate of the infection of bacterial Lform in ACG group and in CGS group was 806% and 767% respectively.The expression of P21 and P53 were significantly higher in Lform infection positive group than that in negative group(p<0.05).the results indicate that Lform infection was correlated with ACG,CGS and overexpression of P21 and P53.It suggests that ras gene and P53 gene mutation may be related with Lform infection.Bacterial Lform infection may be one of the important factor leading to the ACG.