摘要
目的用流式细胞仪检测壳聚糖及其衍生物对HaCaT细胞膜电位的影响。方法合成不同取代度的N-三甲基壳聚糖(TMC)和N-羧甲基壳聚糖(MCC);用纯水作对照,运用荧光分子探针DiBAC4(3)标记HaCaT细胞膜电位,流式细胞仪检测壳聚糖及其衍生物处理之后细胞膜电位的变化情况。结果与纯水组比较,壳聚糖及其衍生物都能显著降低细胞膜电位,其中,TMC20组作用最明显,TMC40组作用最微弱。结论壳聚糖及其衍生物降低细胞膜电位,可能是其具备透皮吸收促进作用的原因之一。
OBJECTIVE To study the effect ofchitosan and its derivatives on membrane potentials of HaCaT cells using flow cytometer. METHODS N-trimethyl chitosan （TMC） with different degree of quaternization and N-carboxymethylchitosan （MCC） were synthesized from chitosan. HaCaT cells were fluorescent labeled with DiBAC4（3） and the changes of membrane potentials were measured by flow cytometer. RESULTS Compared with pure water, all of chitosan and its derivatives decreased HaCaT cells membrane potentials significantly, and the effect of TMC20 was the most significant while that of TMC40 was the weakest. CONCLUSION The decrease effect of chitosan and its derivatives on HaCaT cells membrane potentials might be one of reasons of their skin penetration enhancment.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第14期1063-1066,共4页
Chinese Pharmaceutical Journal
基金
湖北省自然科学基金资助项目(2005ABA107)
