人乳头瘤病毒DNA在人喉癌细胞中表达的研究

The Expression of Human Papillomavirus DNA in Human Laryngeal Carcinoma Cells

为研究喉癌与人乳头状瘤病毒(HPV)感染的关系,本研究探讨了HPV在喉癌中的致病作用和基因组型的分布与表达。应用共有引物和多重引物PCR的方法,对160例喉不同病变的新鲜组织标本,进行HPV6、11、16、18、31、33、35、42、58共9型HPVsDNAs感染的检测。结果在喉癌组HPV感染的阳性率为49.3％(35/71),喉癌颈转移淋巴结组为22.7％(5/22),喉癌前病变组为11.8％(2/17),声带息肉组为6.7％(2/30),癌周正常喉组织为0％(0/20)。HPV DNA型别分布在喉癌中以HPV16、18型为主,喉良性病变中以HPV6、11型为主。表明喉癌发生发展与HPV感染相关。

In older to study the interrelationship between laryngeal carcinoma and human papillomavirus (HPV). The distribution and expression of genome types of HPV in laryngeal carcinoma were investigated. HPV DNA of 160 cases of fresh tissue samples with different lesions of the larynx was detected by applying consensus primers and multiple primers of polymerase chain reaction (PCR) . The consensus primers used were able to detect 9 types of HPVs DNAs such as HPV6, 11, 16, 18, 31, 33, 34, 42 and 58. The results turned out to be as follows: The rate of positive cases with HPV infection was 49.3% (35/71) for the group of laryneal carcinoma, 22.7% (5/22) for the group of neck metastatic lymphnode, 11.8% (2/17) for precarcinomous lesions and 6.7% (2/30 ) for the group of vocal cord polypus, 20 case of normal laryngeal tissue adjacent to the carcinoma was HPV DNA negative. The results demonstrated that the occurrence and development of laryngeal carcinoma were closely related to HPV infection.