G蛋白竞争性抑制性肽(GCIP-27)抑制野百合碱所诱导的大鼠右心室心肌细胞凋亡

G protein competitive inhibitory peptide (GCIP-27) inhibits the right ventricular cardiomyocyte apoptosis induced by monocrotaline in rats

目的：探讨G蛋白竞争性抑制性肽（GCIF-27）对野百合碱（monocrotaline，MCT）所诱导的右心室心肌细胞凋亡的影响。方法：SD大鼠随机分为正常组、MCT模型组、GCIP-27低剂量处理组（30μg·kg^-1）和GCIP-27高剂量处理组（90μg·kg^-1）。MCT造模后d1～d21给GCIP-27（ip,bid）干预，d22用右心导管法测右心室收缩压（RVSP）；用4％多聚甲醛固定右心室组织，用TdT介导的缺口末端标记法（TUNEL法）检测右心室心肌细胞凋亡情况；免疫组化法检测右心室心肌细胞Bcl-2及Bax抗原表达。结果：与MCT模型组相比，GCIP-27能显著改善MCT所诱导的RVSP的升高（P〈0．05，P〈0．01）、右心室心肌细胞的凋亡（P〈0．01）、Ba。抗原的高表达（P〈0．01）以及Bcl-2抗原的低表达（P〈0．01）。结论：GCIP-27能抑制MCT所诱导的大鼠右心室心肌细胞凋亡，该效应可能与其降低RVSP，上调Bcl-2抗原表达，以及下调Bax抗原的表达相关。

Objective： To determine the effect of C, protein competitive inhibitory peptide （GCIP-27） on the right ventricular eardiomyoeyte apoptosis induced by monocrotaline （MCT） and the possible mechanism. Methods： Sprague-Dawley rats were randomly divided into 4 groups. Cardiomyocyte apoptosis was induced by MCT, and treated with GCIP-27 （30 or 90μg·kg^-1, ip, bid）. On day 22, right ventricular systolic pressure （RVSP） was measured through right heart catheterization. The apoptosis of right ventricular cardiomyocytes was detected by TdT mediated nick dUTP end-labeling （TUNEL） technique in the paraffin-embedded right ventrieular tissues. The expression of Bcl-2 and Bax antigens in right ventricular tissues was evaluated by immunohistochemical technique. Results ： GCIP-27 significantly inhibited the RVSP （P 〈 0.05 ,P 〈 0.01 ） and reduced the right ventricular eardiomyocyte apoptosis（P 〈 0.01 ）. GCIP-27 markedly improved MCT-induced Bax over-expression and Bcl-2 low-expression in right ventricular cardiomyocytes（P 〈 0.01 ）. Conclusion： GCIP-27 can attenuate MCT-induced apoptosis of right ventricular cardiomyocytes in rats ; the mechanism may be associated with the up-regulation of Bcl-2 and down-regulation of Bax as well as the reduction in RVSP.