HPLC-ELSD法同时测定复方柴术片中柴胡皂苷a、芍药苷与甘草酸的含量

Simultaneous determination of saikosaponin a,paeoniflorin and glycyrrhizic acid in compound prescription Chaizhu tablets by HPLC-ELSD

目的：建立同时测定复方柴术片中柴胡皂苷a、芍药苷和甘草酸含量的HPLC方法，为制剂的质量控制提供有效的分析方法。方法：Kromasil C18柱（250mm×4．6mm，5μm）；流动相组成A：乙腈，B：0．01％甲酸水溶液，梯度洗脱；流速：1．0mL·mmin^-1，柱温：25℃；蒸发光散射检测器参数：漂移管温度100℃，气速2．0L·min^-1。结果：在选定的色谱条件下，柴胡皂苷a、芍药苷和甘草酸与相关物质分离完全。柴胡皂苷a线性范围为0．0434～0．694μg，r=0．9991，平均回收率为94．1％（RSD=1．7％，n=6）。芍药苷线性范围为0．234～3．74μg，r=0．9996，平均回收率为95．2％（RSD=1．5％，n=6）；甘草酸线性范围为0．228～3．64μg，r=0．9993，平均回收率为94．2％（RSD=1．4％，n=6）。结论：本方法简便、准确、专属性强，可用于复方柴术片中柴胡皂苷a、芍药苷和甘草酸的含量测定。

Objective：To develop a HPLC-ELSD method for simultaneous determination of saikosaponin a, paeoniflorin and glycyrrhizic acid in compound prescription Chaizhu tablets for the quality control. Methods： A Kromasil C18 column （250mm×4.6mm,5μm） was used with a mobile phase of acetonitrile-0.01% formic acid in. gradient elution. The flow rate was 1.0 mL.min 1. Evaporative light scattering detector was used. Drift tube temperature was 100℃. The gas flow was 2.0 L·min^-1 Results：Under the established chromatographic conditions, saikosaponin a,paeoniflorin and glycyrrhizic acid were separated completely within 20 min. The stability under various conditions was good. The linearity was obtained over 0. 043 4-0. 694 μg（r = 0. 999 1 ） for saikosaponin a, 0. 234 -3.74μg（ r=0. 999 6 ） for paeoniflorin, and 0. 228 - 3.64μg （ r = 0. 999 3 ） for glycyrrhizic acid, respectively. The average recoveries of saikosaponin a,paeoniflorin and glycyrrhizic acid were 94.1% （ RSD = 1.7% ,n = 6） ,95. 2% （ RSD =1.5%,n=6）and 94.2% （RSD =1.4%,n =6）,respectively. Conclusion：The method was simple,sensitive,accurate and specific, it could be suitable for the simultaneous determination of saikosaponin a,paeoniflorin and glycyrrhizic acid in compound prescription Chaizhu tablets.