脑卒中伴发原发性高血压ACE基因I/D多态性分析

The Relationship between Ischemic Stroke Accompanied with Hypertension and Insertion/deletion Polymorphism of Angiotensinconverting Enzyme Gene

目的为分析脑卒中伴发高血压及原发性高血压患者血管紧张素转换酶基因插入／缺失（ＡＣＥＩ／Ｄ）多态性。方法用ＰＣＲ方法对１１７例缺血性脑卒中（其中８０例伴发高血压）及１００例年龄、性别相匹配的原发性高血压患者、４０例正常人ＡＣＥ基因Ｉ／Ｄ多态性进行检测。结果脑缺血伴发高血压组ＤＤ基因型频率（０．４４）及Ｄ等位基因型频率（０．５５）显著高于高血压组（０．２２和０．４３）及正常人组（０．１５和０．３７），脑缺血组ＤＤ基因型频率（０．３９）和Ｄ等位基因型频率（０．５４）显著高于高血压组及正常人组。原发性高血压组与正常人组相比无显著差别。结论我国汉族人脑卒中患者ＡＣＥ基因及基因型频率增加，与是否合并高血压无关，Ｄ等位基因可能是缺血性脑卒中的易感基因。

Aim\ To analyse insertion/deletion polymorphism of angiotensinconverting enzyme gene in ischemic stroke with hypertension and hypertensives.\ Methods\ A 278 bp I/D polymorphism in intron 16 of the ACE gene was examined by polymorphism chain reaction in a study of 117 ischemic stroke patients(80 cases accompanied with hypertension) and 100 hypertensives and 40 control subjects(age,sex matched each other).\ [WT5 HZ]Results\ The polymorphism of the ACE gene was categorized into three genotypes:two deletion alleles(genotype DD),heterozygous alleles(genotype ID),and two insertion alleles(genotype II).\ The frequencies of DD genotype and D alleles in ischemic stroke patients(including hypertensive patients) were significantly higher than that in hypertensives and control subjects.\ There were no difference between hypertensives and control subjects.\ Conclusion\ The results suggest that the DD genotype is associated with ischemic storke,not with hypertension.\ D alleles may be a susceptible gene to stroke in Chinese.