双侧颈总动脉结扎对大鼠学习记忆功能和海马组织形态学的影响

Influence of Permanent Occlusion of Bilateral Common Carotid Arteries onLearning and Memory Ability and Hippocampal Histomorphology in Rats

目的探讨大鼠双侧颈总动脉永久性结扎后，对学习记忆的影响，及其与海马神经元形态学变化之间的关系。方法将大鼠双侧颈总动脉永久性结扎后３０ｄ和６０ｄ时，分别进行水迷宫实验和组织形态学实验。结果发现该模型动物术后３０ｄ时，出现明显的学习记忆障碍；６０ｄ后，学习记忆能力仍然降低，没有恢复的趋势。从组织形态学实验中，发现海马神经元逐渐死亡（凋亡）的退行性变化。

Objective To investigate the cogenitive impairment and neuropathological damage in hippocam pus of rats following permanent occlusion of bilateral common carotid arteries(2OV).Methods The hippocampal histomorphology was observed and analyzed by an image processor,and learning and memory ability was tested using water maze.Results The time through all pathways in the water maze was prolonged and errors in passing the blind alleys obviously increased 30days after 2VO operation on rats.The learning and memory ability of the 2OV rats was still severely impaired and significant loss of the cells was observed in the CA1 subfield of hippocampus 60 days after the operation.Conclusions the progressive neuronal degeneration following the permanent 2OV is in part involved in chronic cerebral hypoperfusion induced long lasting deficits of learning and memory in rats.This animal models may be advantageous to the evaluation of drugs that prevent and treat vascular dementia.