TIMP-1及MMP-2/-9基因在人胃癌中的过度表达

Overpression of MMP-2/-9 and TIMP-1 gene in human gastric carcinoma─-study with immunohistochemistry and in situ hybridization

目的对41例人胃癌和17例癌旁组织中的金属蛋白酶MMP－2，MMP－9及金属蛋白酶抑制剂TIMP－1基因表达进行了检测；方法应用免疫组织化学（SP）法和原位杂交（cDNA－mRNA）技术对41例胃癌及癌旁组织的金属蛋白酶及其抑制剂进行检测；结果MMP－2及MMP－9蛋白在胃癌及癌旁组织阳性率分别是73．1％（30／41），65．8％（27／41）和22．5％（4／17），41．1％（7／17）。胃癌及癌旁组织的TIMP－1的表达分别是56．9％（23／41）及34．6％（6／17），且MMP－9／TIMP－1的比值与胃癌的侵袭转移呈正相关。一致性检验显示基因的免疫组化和原位杂交检测结果的关系密切（P＜0．05）；结论MMPS基因的过度表达参与了胃癌的发生发展过程，同时证明TIMP基因的表达参与了肿瘤细胞的生物学行为的改变过程，MMPs／TIMP基因的平衡表达及免疫组化和原位杂交是检测胃癌组织侵袭，转移的可靠方法，本实验的结果为建立肿瘤转移的分子标志提供了理论和实验依据。

Purpose To explove the change of expression of metalloproteinase-2/-9 and tissueinhibitor of metalloproteinase-1 in human gastric carcinoma. Methods lmmunohistochemitry (SPmethod) and in situ hybridization (cDNA-mRNA) were used to detect MMP-2/-9 and TIMP-1 gene ex- pression in tissue of human gastric carcinoma (HGC 41 cases) and near non-tumourous gastric (NT 17cases). Results The positive rates of MMP-2/-9 were 73. 1% (30/41), 65. 8% (27/41 ) and 22. 5% (4/17), 41. 1 % (7/17) in HGC and in NT. The positive rates of TIMP-1 was 56. 9 % (23/41) and 34. 6%(6/17) respectively. The expression of MMP-2/-9 and TIMP-1 at protein level was signficantly correla- tive with that at mRNA level. Conclusion These observations suggest a close association of MMP-2/-9 and TIMP-1 gene expressions with gastricinogenesis. Immunotistochehistry (SP method ) on sectionof paraffin embedded tissue is a reliable method for detecting MMP-2/-9 and TIMP-1 gene expression inHGC.[