激光对猪色素上皮细胞间通讯功能的影响

The effect of laser on intercellular communication of cultured porcine retinal pigment epithelial cells

目的：观察激光对培养的猪色素上皮细胞的通讯功能的影响。方法：用不同输出功率的氩激光直接照射融合状态下的色素上皮细胞，然后测定光斑直径周围细胞的胞间通讯功能。结果：设定激光光斑直径为２００μｍ，曝光时间为０．２ｓ，功率输出为０．１Ｗ时对细胞通讯功能无明显影响、０．５Ｗ的激光对细胞通讯功能有轻度的抑制作用、０．９Ｗ、１．８Ｗ、２．７Ｗ的激光对细胞通讯功能则有明显的抑制作用。结论：不同功率的激光光凝对细胞胞间通讯功能影响程度不同。

Objective:To observe the affect of laser on the function of intercellular communication in cultured pig ertinal pigment epithelial (RPE) cells. Methods:Different power of argon lasers were used to coagulated confluent RPE cells, and then the capacity of intercellular communication of RPE cells rounding the laser burn spots was measured. Results:The were not any effects on the degree of intercellular communication under 0.2 second duration, 200um spot size and 0.1W laser dose. 0.5W Laser showed light inhibitor function. While when the laser dosed increased to 0.9、1.8、and 2.7W apparent restrained aftereffect appeared.Conclusion:Different power of lasers have variable effects on the intercellular communication, and the degree of inhibition is closely related to the energy of the laser. 