术中富集骨髓细胞构建组织工程骨成骨能力的研究

Osteogenic effect of tissue-engineered bone constructed by poly-L-lysine-demineralized bone matrix enriched bone marrow cells

目的观察术中富集骨髓千细胞构建的组织工程骨在山羊脊柱横突间融合的成骨效果，为组织工程骨探索一种新型的构建方法。方法将多聚左旋赖氨酸（poly—L—lysine，PLL）修饰于山羊脱钙骨基质（demineralized bone matrix，DBM）制备成基质材料（PLL—DBM）；以山羊横突间隙为植骨模型，观察PLL-DBM富集骨髓细胞构建的组织工程骨（ⅠA组）的成骨能力；对照组为自体髂骨组（ⅠB组）、DBM富集骨髓组（ⅡC组）、空白DBM组（ⅡD组）。于术后第16周取融合段标本行X线片、三维CT及CT值检测和生物力学检测，对比分析、评价其成骨能力。结果X线表现：ⅠA组、ⅠB组融合范围基本相同，明显较ⅡC组宽，ⅡD组基本无融合。三维CT：ⅠA组的CT值为（696．76±102．75）HU，ⅠB组的CT值为（766．03±69．24）HU，二者差异无统计学意义（P〉0．05），均明显较ⅡC组高（P〈0．05），ⅡC组CT值较ⅡD组高（P〈0．01）。生物力学性能：ⅠA、ⅠB两组最大载荷、抗弯强度比较差异均无统计学意义（P〉0．05），ⅠA组较ⅡC组高（P〈0．01，0．05），ⅠB组较ⅡC组高（P〈0．01），ⅡC组较ⅡD组高（P〈0．01）。结论PLL—DBM术中富集骨髓干细胞快速构建的组织工程骨是一种理想的组织工程骨，其成骨能力与自体髂骨相当。

Objective To observe the osteogcnic effect of tissue-engineered bone constructed by poly-L-lysine-demineralized bone matrix （PLL-DBM） enriched hone marrow stem cells in the space of goat transverse process bone fusion model and explore a new tissue-engineered bone construction method. Methods PLL was used to decorate goat DBM to prepare a matrix material （PLL-DBM）. The osteogenic effect of tissue-engineered bone constructed by PLL-DBM enriched bone marrow cells （ Group Ⅰ A） was detected in goat lumbar intertransvcrse graft bone model; autogeuous iliac bone （Group ⅠB） , DBM enriched bone marrow （Group ⅡC） and DBM （Group ⅡD） were used as controls. The osteogenesis of the bones in the fused segments of four groups were compared and evaluated by X-ray, three-dimensional CT, CT value testing and biomechanical testing. Results The results of X-ray showed that the fusion ranges in groups ⅠA and ⅠB were basically the same, which were significantly wider than that in Group Ⅱ, with no fusion detected in Group ⅡD. The CT value was （696.76 ±10275 ） HU in Group Ⅰ A and （766.03 ±69.24） HU in Group B, which were significantly higher than that in Group Ⅱ C （P 〈 0. 05）, but there was no statistical difference in CT value between Groups Ⅰ A and ⅠB （ P 〉 0.05 ）. The CT value in Group Ⅱ C was significantly higher than in Group ⅡD （P 〈0.01 ）. There was no statistical difference between Groups ⅠA and ⅠB in the maximum load and bending strength （ P 〉 0.05 ）. The maximum load and bending strength in Groups Ⅰ A and ⅠB were significantly higher than that in Group Ⅱ C （P 〈 0.05 ）, and the two indices in Group Ⅱ C were significantly higher than that in Group Ⅱ D （ P 〈0. 01）. Conclusion Tissue-engineered bone constructed by PLL-DBM enriched bone marrow cells is an ideal tissue engineered bone and its osteogenic potential is similar to that of autologous bone.