核移植影响因素研究进展

The Progress of Research on the Influencing Factor of Nuclear Transfer

核移植技术是近年发展起来的细胞克隆技术,核移植的方法有电融合法、PEG介导法和微注射法。影响核移植成功与否的参数包括核供体细胞的来源、胞质体来源细胞的发育阶段和核供体细胞周期的同步性。不同的核供体细胞的收集方法也可能对核移植效率产生影响。核移植后会出现基因程序重编,重组胚重新获得多能性,开始新的胚胎发育。

Nuclear transfer （NT） is a new cloning technology developed in recent years. NT methods consist of electrofusion, NT mediated by polyethylene glycol （PEG） and microinjection. The success of somatic nuclear transfer depends on the source of donor nucleus, developmental stage of recipient eytoplasts, cell cycle synchrony of donor nucleus. Different methods of harvesting cells have effect on the efficiency of NT. The somatic nucleus will be repro-grammed after NT and will restore a totipotent state in order to undergo development.