伽玛刀治疗后P53蛋白和PCNA蛋白在复发胶质母细胞瘤中表达的变化及其临床意义

Variance and Significance of Expression of P53 and PCNA Proteins in Recurrent Glioblastoma after Gamma Knife

目的:初步探讨伽玛刀治疗前后复发胶质母细胞瘤中P53及PCNA蛋白表达的变化及其临床意义。方法:用免疫组化SP法检测29例复发胶质母细胞瘤患者(伽玛刀刀治疗组12例,非伽玛刀治疗组17例)在初发和复发的肿瘤组织中P53蛋白和PCNA蛋白的表达。结果:两组患者在性别,年龄,肿瘤部位及大小构成上差异无统计学意义(P>0.05);两组的复发时间的差异有统计学意义(P=0.0409<0.05);在伽玛刀治疗组P53及PCNA蛋白在复发胶质母细胞瘤中表达明显降低(p53,t=3.915,P=0.02<0.05;PCNA,t=2.962,P=0.013<0.05);非伽玛刀治疗组p53及PCNA蛋白在复发胶质母细胞瘤中表达明显增加(p53,t=-5.926,P=0.000<0.05;PCNA,t=-5.160,P=0.000<0.05);P53及PCNA蛋白在伽玛刀治疗组和非伽玛刀治疗组的表达变化有统计学意义(p53,t=-5.577,P=0.000<0.05.PCNA,t=-5.542,P=0.000<0.05);在伽玛刀治疗组及非伽玛刀治疗组,P53蛋白和PCNA蛋白的阳性表达率不存在明显的相关性(伽玛刀治疗组,r=-0.085,P=0.792>0.05.非伽玛刀治疗组,r=0.450,P=0.07>0.05)。结论:P53及PCNA蛋白的异常表达与胶质母细胞瘤的复发有关,伽玛刀治疗胶质母细胞瘤瘤可能通过抑制P53及PCNA蛋白表达而起作用。

Objective： To investigate the variance and significance of expression of P53 and PCNA proteins in recurrent glioblastoma after gamma knife treatment.Methods： The expression of P53 protein and PCNA proteins in the primary and recurrent glioblastoma tissues of 29 recurrent glioblastoma patients（there are 12 patients in the gamma knife treatment group and 17 patients in the non-gamma knife treatment group）was detected by immunohistochemical staining.Results： There was no significant difference in patient＇s gender,age,tumor site and size between two group while the recurrent time had significant difference;In gamma knife treatment group,the expression of P53 protein and PCNA proteins decreased,while ascended in non-gamma knife treatment group（gamma knife treatment group： P53,t=3.915,P=0.02〈0.05;PCNA,t=2.962,P=0.013〈0.05.non-gamma knife treatment group： P53,t=-5.926,P=0.000〈0.05;PCNA,t=-5.160,P=0.000〈0.05）.The variance of expression of P53 and PCNA proteins had significant difference between two groups（P53,t=-5.577,P=0.000〈0.05.PCNA,t=-5.542,P=0.000〈0.05）.The expression of P53 protein and PCNA protein had no visible dependability in two group（gamma knife treatment group,r=-0.085,P=0.792〉0.05.non-gamma knife treatment group,r=0.450,P=0.07〉0.05）.Conclusion： The abnormal expression of P53 and PCNA proteins was relevant with recurrent glioblastoma.The expression of P53 protein and PCNA protein was inhibited in glioblastoma after gamma knife treatment.